Culturable soil microbial biodiversity in post-mining areas of the Konin lignite mine and its potential for ecosystem restoration
DOI:
https://doi.org/10.12775/EQ.2026.009Abstract
Post-mining areas represent strongly transformed environments that provide unique conditions for studying microbial diversity and ecological succession. This study aimed to characterize the bacterial and fungal communities isolated from soil samples collected in the post-mining landscapes of the Konin Lignite Mine (central Poland). Cultivable microorganisms were isolated and identified using molecular methods based on 16S rRNA (bacteria) and ITS (fungi) gene sequencing. Taxonomic classification was conducted using BLAST and SILVA (bacteria) and UNITE (fungi) databases. Identified taxa included bacteria from genera Pseudomonas, Bacillus, Micrococcus, and Stutzerimonas, and fungi from genera Cladosporium, Trichoderma, and Penicillium. Many of these microorganisms exhibit traits relevant to bioremediation and supporting plant growth in degraded soils. Our findings highlight the ecological potential of soil microbiota in post-industrial ecosystems and their importance in restoration efforts.
References
Adamczyk, W., et al. (2014). Environmental degradation of post-mining areas in Poland. Pol. J. Environ. Stud., 23(5), 1601–1610.
Chen, J., et al. (2024). Soil microbial diversity and nitrogen cycling in degraded ecosystems. Applied Soil Ecology, 196, 105260.
da Silva, R., et al. (2022). The genus Cladosporium: A rich source of diverse and bioactive products. Microorganisms, 10(5), 966. https://doi.org/10.3390/microorganisms10050966.
Dubey, M. K., & Jensen, D. F. (2021). Clonostachys rosea — biology and potential use as a biocontrol agent. Frontiers in Microbiology, 12, 654321. https://doi.org/10.3389/fmicb.2021.654321.
Dusengemungu, L., et al. (2022). Metal tolerance and bioremediation potential of Cladosporium species. Mycology, 13(1), 12–24.
Ezeokoli, O.T., et al. (2020). Plant growth-promoting bacteria in degraded mining soils. Journal of Hazardous Materials, 398, 122771.
Fashola, M.O., et al. (2016). Heavy metal pollution from mining: A review. Ecotoxicology and Environmental Safety, 126, 213–227.
Feng, Y., et al. (2019). Soil microbial community and restoration strategies. Science of the Total Environment, 659, 473–484.
Harman, G. E., Howell, C. R., Viterbo, A., Chet, I., & Lorito, M. (2004). Trichoderma species — opportunistic, avirulent plant symbionts. Nature Reviews Microbiology, 2(1), 43–56. https://doi.org/10.1038/nrmicro797.
Jakubus, M., Gajewski, P., & Kaczmarek, Z. (2013). Właściwości fizykochemiczne i chemiczne poziomów wierzchnich wybranych gleb zlokalizowanych w sąsiedztwie planowanej odkrywki węgla brunatnego „Tomisławice”. Ochrona Środowiska, 15, 2232–2248.
Kivinen, S. (2017). Post-mining land use and ecological recovery. Ecological Engineering, 101, 145–152.
Murphy, C.D., et al. (2021). Biodegradation of alkanes by environmental bacteria. Biotechnology Advances, 46, 107653.
Neina, D. (2019). Soil pH and microbial communities in disturbed lands. Applied Soil Ecology, 141, 10–18.
Shafi, J., et al. (2017). Bacterial resilience in metal-contaminated soils. Frontiers in Microbiology, 8, 1935.
Singh, R., et al. (2020). Microbial bioremediation of contaminated soils: A review. Environmental Pollution, 266, 115345.
Stamm, W. E., & et al. (2006). Biology of Pseudomonas stutzeri. Clinical Microbiology Reviews, 19(2), 293–314. https://doi.org/10.1128/CMR.19.2.293 314.2006.
Tomczyk-Żak, K., et al. (2017). Microbial colonization in lignite mine environments. Microbial Ecology, 73(4), 964–976.
Tripathi, P., et al. (2013). Role of Trichoderma in agriculture and bioremediation. Environmental Monitoring and Assessment, 185, 4757–4770.
Wanga, L., et al. (2023). Biodegradation and denitrification potential of Stutzerimonas spp. Journal of Applied Microbiology, 134(2), 196–208.
White, T.J., Bruns, T., Lee, S., Taylor, J.W. (1990). Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J. (eds). PCR Protocols: A Guide to Methods and Applications. Academic Press, 315–322.
Wulandari, M., et al. (2024). Arbuscular mycorrhizal fungi and soil recovery in mining areas. Land Degradation & Development, 35(1), 89–102.
Yan, Q., Li, Y., & Xie, J. (2021). Isolation and identification of an efficient aerobic denitrifying Pseudomonas stutzeri strain and characterization of its nitrite degradation. Catalysts, 11(10), 1214. https://doi.org/10.3390/catal11101214.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Magdalena Lewandowska, Agnieszka Richert, Natalia Hejda

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Stats
Number of views and downloads: 3
Number of citations: 0