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Ecological Questions

Priming of Brassica napus L. seeds with aqueous extract from mistletoe (Viscum album L.) boosts the content of photosynthetic pigments
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  • Priming of Brassica napus L. seeds with aqueous extract from mistletoe (Viscum album L.) boosts the content of photosynthetic pigments
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Priming of Brassica napus L. seeds with aqueous extract from mistletoe (Viscum album L.) boosts the content of photosynthetic pigments

Authors

  • Grażyna B. Dąbrowska Department of Genetics, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń https://orcid.org/0000-0002-8300-3009
  • Magdalena Michalak
  • Marcel Antoszewski
  • Dariusz Kamiński Uniwersytet Mikołaja Kopernika w Toruniu https://orcid.org/0000-0001-7842-6704
  • Agnieszka Mierek-Adamska Uniwersytet Mikołaja Kopernika w Toruniu https://orcid.org/0000-0001-8247-7328

DOI:

https://doi.org/10.12775/EQ.2024.056

Keywords

mistletoe, Brassica napus, plant growth, antibacterial properties, seed treatment, plant extracts, HPMO

Abstract

The increasing occurrence and severity of drought periods due to climate change have a negative impact on crop yield worldwide. In the face of a rising human population, this poses a severe threat to food security. Natural products such as plant extracts have many uses, but the studies about their potential usage in seed pre-sowing treatments have been limited. In this study, we evaluated the potential of Viscum album L. extract as a Brassica napus L. seed coating and seed priming agent. Moreover, its potential to limit the growth of commonly occurring foodborne pathogens was verified. The results showed that seed treatments with mistletoe macerate did not significantly affect hypocotyl and root length and biomass. Chlorophyll and carotenoid content of the plants treated with mistletoe extracts increased – with the best results obtained for the seeds humidified with 20% extract and 50% for priming. V. album extract exhibits weak antibacterial properties towards Escherichia coli and Salmonella typhimurium and does not show antibacterial properties against Staphylococcus aureus. This study points to the new possible use of plant extracts as an ecological way for seed pre-sowing treatment.

References

Adinde J.O., Omeje T.E., Uche O.J. & Agu C.J., 2020, Impact of hydropriming duration on seed germination and emergence indices of sweet basil. Journal of Agricultural Science and Practice 5(1): 1–7. https://doi.org/10.31248/JASP2019.177

Agbo M.O., Odimegwu D.Ch., Okoye F.B.Ch. & Osadebe P.O., 2017, Antiviral activity of Salidroside from the leaves of Nigerian mistletoe (Loranthus micranthus Linn) parasitic on Hevea brasiliensis against respiratory syncytial virus. Pakistan Journal of Pharmaceutical Sciences 30(4): 1251–1256.

Amutenya A., Kwembeya E., Shikangalah R. & Tsvuura Z., 2023, Photosynthesis, chlorophyll content and water potential of a mistletoe-host pair in a semi-arid savanna. South African Journal of Botany 163: 311–315. https://doi.org/10.1016/j.sajb.2023.10.053

Anjum S.A., Xie X.Y., Wang L.C., Saleem M.F., Man C. & Lei W., 2011, Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research 6: 2026–2032. Doi: 10.5897/AJAR10.027

Awad D.A., Masoud H.A. & Hamad A., 2024, Climate changes and food-borne pathogens: the impact on human health and mitigation strategy. Climatic Change 177, 92. https://doi.org/10.1007/s10584-024-03748-9

Bennett S.D., Sodha S.V., Ayers T.L., Lynch M.F., Gould L.H. & Tauxe R.V., 2018, Produce-associated foodborne disease outbreaks, USA, 1998–2013. Epidemiology and Infection 11: 1397–1406. Doi:10.1017/S0950268818001620

Belgüzar S., Şin B., Eroğlu Z., Kadıoğlu İ. & Yanar Y., 2020, The effect of Common Mistletoe (Viscum album L.) Extract from Different Host Plants, on some Plant Pathogenic Bacteria. Turkish Journal of Agriculture - Food Science and Technology 8(sp1): 80–84. https://doi.org/10.24925/turjaf.v8isp1.80-84.3987

Borges C.E., Veloso R., da Conceição C.A., Mendes D.S., Cabral N.R., Shabani F., Tehrany M.S., Nery M.C. & da Silva R.S., 2023, Forecasting Brassica napus production under climate change with a mechanistic species distribution model. Scientific Reports 13, 12656. Doi: 10.1038/s41598-023-38910-3

Chakraborty P. & Dwivedi P., 2021, Seed priming and its role in mitigating heat stress response in crop response. Journal of Soil Science and Plant Nutrition 21: 1718–1734. https://doi.org/10.1007/s42729-021-00474-4

Chahar M., Kroupitski Y., Gollop R., Belausov E., Melotto M. & Sela-Saldinger S., 2021, Determination of Salmonella enterica leaf internalization varies substantially according to the method and conditions used to assess bacterial localization. Frontiers in Microbiology 12, 622068. Doi:10.3389/fmicb.2021.622068

Chekol H., Bezuayehu Y., Warkineh B., Shimber T., Mierek-Adamska A., Dąbrowska G. & Degu A., 2023, Unraveling drought tolerance and sensitivity in coffee genotypes: insights from seed traits, germination, and growth-physiological responses. Agriculture 13(9), 1754. Doi:10.3390/agriculture13091754

Dąbrowska G., Garstecka Z., Trejgell A., Dąbrowski H.P, Konieczna W. & Szyp-Borowska I., 2021, The impact of forest fungi on promoting growth and development of Brassica napus L. Agronomy 11(12), 2475. Doi:10.3390/agronomy11122475

Dąbrowska G.B., Dąbrowski H.P. & Richert A., 2020, Identification of the Ipomoea nil cDNA clone encoding protein with one transmembrane domain by differential display PCR. Ecological Questions 31(2): 71–78. Doi:10.12775/EQ.2020.015

Dąbrowska G., Turkan S., Tylman-Mojżeszek W. & Mierek-Adamska A., 2021a, In silico study of the RSH (RelA/SpoT homologs) gene family and expression analysis in response to PGPR bacteria and salinity in Brassica napus. International Journal of Molecular Sciences 22(9), 10666. Doi:10.3390/ijms221910666

Iriarte A., Rieradevall J. & Gabarrell X., 2010, Life cycle assessment of sunflower and rapeseed as energy crops under Chilean conditions. Journal of Cleaner Production 18(4): 336–345. Doi:10.1016/j.jclepro.2009.11.004

Ekhaise F.O., Ofoezie V.G. & Enobakhare D.A., 2010, Antibacterial properties and preliminary phytochemical analysis of methanolic extract of mistletoe (Tapinanthus bangwensis) Bayero. Journal of Pure and Applied Sciences 3(2): 65–68.

Elouaer M.A. & Hannachi C., 2012, Seed priming to improve germination and seedling growth of safflower (Carthamus tinctorius) under salt stress. EurAsian Journal of BioSciences 6: 76–84. Doi:10.5053/ejobios.2012.6.0.9

Findura P., Hara P., Szparaga A., Kocira S., Czerwińska E., Bartoš P., Nowak J. & Treder K., 2020, Evaluation of the Effects of Allelopathic Aqueous Plant Extracts, as Potential Preparations for Seed Dressing, on the Modulation of Cauliflower Seed Germination. Agriculture 10(4), 122. Doi:10.3390/agriculture10040122

Garstecka Z., Antoszewski M., Mierek-Adamska A., Krauklis D., Niedojadło K., Kaliska B., Hrynkiewicz K. & Dąbrowska G., 2023, Trichoderma viride colonizes the roots of Brassica napus L., alters the expression of stress-responsive genes, and increases the yield of canola under field conditions during drought. International Journal of Molecular Sciences 24(20), 15349. Doi:10.3390/ijms242015349

Hammer O., Harper D.A. & Ryan P.D., 2001, PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4(1): 1–9.

Horneber M., Bueschel G., Huber R., Linde K. & Rostock M., 2008,

Mistletoe therapy in oncology. Cochrane Database of Systematic Reviews. Doi:10.1002/14651858.cd003297.pub2

Hussain M.A., Khan M.Q., Hussain N. & Habib T., 2011, Antibacterial and antifungal potential of leaves and twigs of Viscum album L. Journal of Medicinal Plants Research 5(23): 5545–5549.

Ilyas S., 2006, Seed Treatments Using Matriconditioning to Improve Vegetable Seed Quality. Bull Agronomy Journal 34: 124–132.

Irobi O.N., 1992, Activities of Chromolaena odorata (composital) leaf extract against Pseudomonas auruginosa and Streptococcus faecalis. Journal of Ethnopharmacology 37: 81–83.

Jacob C. & Melotto M., 2020, Human pathogen colonization of lettuce dependent upon plant genotype and defense response activation. Frontiers in Plant Science 10, 1769. Doi: 10.3389/fpls.2019.01769

Johnson J.M. & Choinski J.S., Jr., 1993, Photosynthesis in the Tapinanthus – Diplorhynchus mistletoe-host relationship. Annals of Botany 72: 117–122. Doi:10.1006/anbo.1993.1088

Kaur S., Gupta A.K. & Kaur N., 2002, Effect of osmo- and hydropriming of chickpea seeds on seedling growth and carbohydrate metabolism under water deficit stress. Plant Growth Regulation 37: 17–22. Doi: 10.1023/A:1020310008830

Kelter G., Schierholz J.M., Fischer I.U. & Fiebig H.H., 2007, Cytotoxic activity and absence of tumor growth stimulation of standardized mistletoe extracts in human tumor models in vitro. Anticancer Research 27(1A): 223–233.

Khan S., Ibrar D., Bashir S., Rashid N., Hasnain Z., Nawaz M., Al-Ghamdi A.A., Elshikh M.S., Dvorackova H. & Dvoracek J., 2022, Application of Moringa Leaf Extract as a Seed Priming Agent Enhances Growth and Physiological Attributes of Rice Seedlings Cultivated under Water Deficit Regime. Plants 11(3), 261. https://doi.org/10.3390/plants11030261

Konieczna W., Mierek-Adamska A., Chojnacka N., Antoszewski M., Szydłowska-Czerniak A. & Dąbrowska G.B., 2023a, Characterization of the metallothionein gene family in Avena sativa L. and the gene expression during seed germination and heavy metal stress. Antioxidants 12, 1865. https://doi.org/10.3390/antiox12101865

Konieczna W., Warchoł M., Mierek-Adamska A., Skrzypek E., Waligórski P., Piernik A., Dąbrowska G., 2023b, Changes in physio-biochemical parameters and expression of metallothioneins in Avena sativa L. in response to drought. Scientific Reports 13(1), 2486. Doi:10.1038/s41598-023-29394-2

Kowalska G. Kowalski R.; Hawlena J. & Rowinski R., 2020, Seeds of oilseed rape as an alternative source of protein and minerals. Journal of Elementology 25: 513–522.

Lin L., Allemekinders H., Dansby A., Campbell, L., Durance-Tod S., Berger A. & Jones P.J., 2013, Evidence of health benefits of canola oil. Nutrition Reviews 71(6): 370–385. Doi:10.1111/nure.12033

Lichtenthaler H.K. & Wellburn A.R., 1983, Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions 11: 591–592.

Luna-Guevara J.J., Arenas-Hernandez M.M.P., de la Peńa C.M., Silva J.L. & Luna-Guevara M.L., 2019, The role of pathogenic E. coli in fresh vegetables: behavior, contamination factors, and preventive measures. International Journal of Microbiology Nov 26: 2019, 2894328. Doi:10.1155/2019/2894328

Macarsin D., Patel J., Bauchan G., Giron J.A. & Ravishankar S., 2013, Effect on spinach cultivar and bacterial adherence factors on survival of Escherichia coli O157:H7 on spinach leaves. Journal of Food Protection 76(11): 1829–1837. Doi:10.4315/0362-028x.jfp-12-556

Mapfumari S., Nogbou N.-D., Musyoki A., Gololo S., Mothibe M. & Bassey K., 2022, Phytochemical screening, antioxidant and antibacterial properties of extracts of Viscum continuum E. Mey. ex Sprague, a South African mistletoe. Plants-Basel 11(16), 2094. https://doi.org/10.3390/plants11162094

Mathiasen R.L., Nickrent D.L., Shaw D.C. & Watson D.M., 2008, Mistletoes: Pathology, systematics, ecology, and management. Plant Disease 92(7): 988–1006. Doi:10.1094/pdis-92-7-0988

Matsubara S., Gilmore A.M., Ball M.C., Anderson J.M. & Osmond C.B., 2002, Sustained downregulation of photosystem II in mistletoes during winter depression of photosynthesis. Functional Plant Biology 29: 1157–1169.

Meinzer F.C., Woodroof D.R. & Shaw D.C., 2004, Integrated responses of hydraulic architecture, water and carbon relations of western hemlock to dwarf mistletoe infection. Plant, Cell and Environment 27(7): 937–946. Doi:10.1111/j.1365-3040.2004.01199.x

Mierek-Adamska A., Kotowicz K., Goc A., Boniecka J., Berdychowska J. & Dąbrowska G.B., 2019, Potential involvement of rapeseed (Brassica napus L.) metallothioneins in the hydrogen peroxide-inducated regulation of seed vigour. Journal of Agronomy and Crop Science 205(6): 598–607. https://doi.org/10.1111/jac.12361

Mogren L., Windstam S., Boqvist S., Vågsholm I., Söderqvist K., Rosberg A.K., Linden J., Mulaosmanovic E., Karlsson M., Uhlig E., Hakansson A. & Alsanius B., 2018, The hurdle approach – a holistic concept for controlling food safety risks associated with pathogenic bacterial contamination of leafy green vegetables – review. Frontiers in Microbiology 9, 1965. Doi: 10.3389/fmicb.2018.01965

Najem E., Chabuck A.A., Al.-Saigh R., Hindi N.K.K. & Kadhum S., 2022, Pathogenic and antimicrobial properties of aquatic extracts of Viscus album and Apium graveolens. Asian Journal of Plant Sciences 21(4): 629–636. Doi:10.3923/ajps.2022.629.636

Oei S.L., Thronicke A. & Schad F., 2019, Mistletoe and immunomodulation: Insights and implications for anticancer therapies. Evidence-Based Complementary and Alternative Medicine 5893017: 6. Doi:10.1155/2019/5893017

Ostrowska A, Kozłowska M., Rachwał D., Wnukowski P., Nebesny E. & Rosicka-Kaczmarek J., 2018, Rapeseed protein-fibre concentrate: chemical composition and functional properties. Food. Science. Technology. Quality, 25, 4(117): 86–99. Doi: 10.15193/zntj/2018/117/261

Osburn R.M. & Schroth M.N., 1989, Effect of osmopriming sugar beet deed on exudation and subsequent damping-off caused by Pythium ultimum. Phytopathology 78: 1246–1250.

Orhue P.O., Edomwande E.C., Igbinosa E., Momoh A.R.M. & Asekomhe O.O., 2014, Antibacterial activity of extracts of mistletoe (Tapinanthus dodoneifollus (Dc) Dancer) from cocoa tree (Theobrama cacao). International Journal of Herbs Pharmacology Research 3: 24–29.

Paparella S., Araujo A.S., Rossi G., Wijayasinghe M., Carbonera D. & Balestrazzi A., 2015, Seed priming: state of the art and new perspectives. Plant Cell Reports 34: 1281–1293. Doi: 10.1007/s00299-015-1784-y

Parera C.A. & Cantliffe D.J., 1994, Presowing seed priming. Horticultural Reviews 16: 109–141. Doi:10.1002/9780470650561.ch4

Pietrzak W. & Nowak R., 2021, Impact of Harvest Conditions and Host Tree Species on Chemical Composition and Antioxidant Activity of Extracts from Viscum album L. Molecules 26, 3741. Doi: 10.3390/molecules26123741

Rigling A., Eilmann B., Koechli R. & Dobbertin M., 2010, Mistletoe-induced crown degradation in Scots pine in a xeric environment. Tree Physiology 30(7): 845–852. Doi:10.1093/treephys/tpq038

Roy B., Sarker B.C., Ali M.R., Das S.R. & Sayed A.S., 2012, Seed germination and seedling growth of two vegetables in responses to aqueous extract of four herbal plant leaves. Journal Environmental Science & Natural Resources 5(1): 141–150. https://doi.org/10.3329/jesnr.v5i1.11569

Saeidnia S. & Gohari A. R., 2012, Importance of Brassica napus as a medicinal food plant. Journal of Medicinal Plants Research 6(14): 2700–2703. doi: 10.5897/JMPR11.1103

Singh G.P. & Chaudhary H.B., 2006, Selection parameters and yield enhancement of wheat (Triticum aestivum L.) under different moisture stress condition. Asian Journal of Plant Sciences 5(5): 894–898.

Saini S., Raj K., Saini A.K., Chugh R.K., Lai M. & Bhambhu M.K., 2023, Efficacy of plant extracts in growth promotion and onion purple blotch management: Unveiling metabolite fingerprinting of promising neem leaf extracts through GC MS. European Journal of Plant Pathology, 2023. https://doi.org/10.1007/s10658-023-02810-z

Shah S.S., Rehman Y.U., Iqbal A., Rahman Z.U., Zhou B., Peng M. & Li Z., 2017, Phytochemical Screening and Antimicribial Activities of Stem, Leaves and Fruit Extracts of Viscum album L. Journal of Pure and Applied Microbiology 11(3): 1337–1349. https://doi.org/10.22207/JPAM.11.3.14

Staniak M. & Baca E., 2018, The effect of drought stress on chlorophyll fluorescence indicators in alfalfa (Medicago x varia Martyn), red clover (Trifolium pratense L.) and white clover (Trifolium repens L.). Grassland Science in Poland 21: 127–138.

Stawski K., Dąbrowska G. & Goc A., 2008, Identification of cDNA and characterization of the OHP2 (One Helix Protein 2) gene of Pharbitis nil Choisy. Advances of Agricultural Sciences Problem Issues 524: 521–528.

Stypiński P., 1997, Biology and ecology of the European mistletoe (Viscum album, Viscaceae) in Poland. Fragmenta Floristica et Geobotanica Series Polonica, Suppl. 1: 3–115.

Szymańska S., Dąbrowska G.B., Tyburski J., Niedojadło K., Piernik A. & Hrynkiewicz K., 2019, Boosting the Brassica napus L. tolerance to salinity by the halotolerant strain Pseudomonas stutzeri ISE12. Environmental and Experimental Botany. 163: 55–68. https://doi.org/10.1016/j.envexpbot.2019.04.007

Taherkhani T., Rahmani N. & Pazoki A., 2013, The effect of hydro-priming on germination of mustard seeds under draught stress conditions. Life Science Journal 10(3s): 392–395.

Tarfa F.D., Obodozie O.O., Mshelia E., Ibrahim K. & Temple V.J., 2004, Evaluation of phytochemical and antimicrobial properties of leaf extract of Tapinanthus sessilifolius (P. Beauv) van Tiegh. Indian Journal of Experimental Biology 42: 326–329.

Turkan S., Mierek-Adamska A., Kulasek M., Konieczna W.B. & Dąbrowska G.B., 2023, New seed coating containing Trichoderma viride with anti-pathogenic properties. PeerJ 11, e15392. https://doi.org/10.7717/peerj.15392

Turker A., Yildirim A. & Karakas F., 2012, Antitumor and antibacterial activities of Viscum album L. grown on different host trees. Spatula DD 2(4): 229–236.

Varga I., Poczai P., Tiborcz V., Aranyi N.R., Baltazár T., Bartha D., Pejchal M. & Hyvönen J., 2014, Changes in the distribution of european mistletoe (Viscum album) in Hungary during the last hundred years. Folia Geobotanica 49(4): 559–577. Doi:10.1007/s12224-014-9193-5

Waller F., Achatz B., Baltruschat H, Fodor J., Becker K., Fischer M., Heier T., Huckelhoven R., Neumann C. & Von-Wettstein D., 2005, The endophytic fungus Piriformis indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield. Proceedings of the National Academy of Sciences 102: 13386–13391. DOI: 10.1073/pnas.0504423102

Wrotek S., Skawiński R. & Kozak W., 2014, Immunostimulatory properties of mistletoe extracts and their application in oncology. Advances in Hygiene and Experimental Medicine 68: 1216–1224. Doi: 10.5604/17322693.1126850

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2024-09-22

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DĄBROWSKA, Grażyna B., MICHALAK, Magdalena, ANTOSZEWSKI, Marcel, KAMIŃSKI, Dariusz and MIEREK-ADAMSKA, Agnieszka. Priming of Brassica napus L. seeds with aqueous extract from mistletoe (Viscum album L.) boosts the content of photosynthetic pigments. Ecological Questions. Online. 22 September 2024. Vol. 35, no. 4, pp. 175-184. [Accessed 1 July 2025]. DOI 10.12775/EQ.2024.056.
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Copyright (c) 2024 Grażyna B. Dąbrowska, Magdalena Michalak, Marcel Antoszewski, Dariusz Kamiński, Agnieszka Mierek-Adamska

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