Studies of the silkworm enzyme activity and their correlations with economic variables
DOI:
https://doi.org/10.12775/EQ.2023.018Keywords
Fifth instar, Haemolymph, Silkworm, Textiles, activities of succinate, glutamate dehydrogenases, Peak valueAbstract
The experiment was conducted to analyse the larval performance and economic traits of bivoltine silkworm breeds of silkworm (SK-1, SK-6, SK-22, SK-28, SK-33, CSR4, CSR2, NB4D2, DUN6 and APS4) during spring season. The haemolymph total protein, succinate and gultamate dehydrogenase activities were estimated and their correlation with economic traits were also worked out. The results of the study confirmed that among ten bivoltine silkworm breeds, highest haemolymph SDH activity of 3.47 µmoles/ml/mgprotein/min was recorded in the silkworm breed SK1 and least SDH activity of 1.58 µmoles/ml/mg protein/min was recorded in the breed APS4. The highest peak of succinate dehydrogenase activity of 2.65 µmoles/ml/mg protein/min was observed on 7th day of the 5th instar and lowest peak of succinate dehydrogenase activity of 2.39 µmoles/ml/mg protein/min was observed on 4th day of the 5th instar. GDH activity of 0.46 µmoles/ml/mg protein/min was recorded highest in the silkworm breed SK1 and lowest of 0.15 µmoles/ml/mg protein/min was recorded in silkworm breed APS4. The highest peak of haemolymph GDH of 0.36 µmoles/ml/mg protein/min was recorded on 7th day of 5th instar and lowest peak of 0.26 µmoles/ml/mg protein/min was recorded on 4th day of 5th instar. The correlational studies revealed that haemolymph total protein, SDH and GDH were found to be positively corelated with yield by weight and number (cocoon), weight of mature larvae, shell weight ,cocoon weight, shell ratio percent, silk productivity, rate of pupation, fecundity, raw silk percentage and length of filament. Thus, the study revealed that silkworm breeds like SK1, SK6, SK22 and SK28 as productive breeds and hence may be used for future breeding programmes for evolution of new robust silkworm breeds
References
Alberts B., Johnson A., Lewis J., Raff M., Roberts K. & Walter P., 2007, Molecular biology of the cell, 5th edition. Chapter 14: Energy Conversion: Mitochondria and Chloroplasts, p. 813-878. Garland Science, New York.
Anonymous, 2003, Package of practices for silkworm rearing and mulberry cultivation in Kashmir. Technical Document, Directorate of Extension Education, Sher Kashmir University of Agricultural Sciences and Technology of Kashmir, 5 pp.
Anonymous, 2019, Annual report. Vision document and road map for cocoon and silk production in Jammu & Kashmir, p. 2-3. Sericulture Development Department.
Anonymous, 2020, Functioning of central silk board and performance of Indian silk industry, p.19-21.
Bannikov V.M., Bachikova A.P., Ushkova G.I. & Fillippovich Y.B., 1982, Study on sub cellular localization of some enzymes in silkworm, Bombyx mori L. eggs. Biokhimiya 47: 1386-1391.
Dasamahapatra A.K., Ray A.K. & Medda A.K., 1990, In vitro demonstration of putative nuclear 3, 5, 3-triiodo thyronine receptor in isolated liver nuclei of singi fish, Heteropneustes fossilis (Bloch). Hormone and Metabolic Research 22: 221-224.
Dickinson W.J. & Sullivan D.T., 1975, Gene enzyme system in Drosophila. Springer, Berlin 11: 12-18.
Etebari K., Mirhodeini S.Z. & Matindoost L., 2005, A study on intraspecific biodiversity of eight groups of silkworms (Bombyx mori) by biochemical markers. Insect Science 12(2): 87-94.
Harper H.A., Rodwell V.W. & Mayes P.A., 1993, Review of physiological chemistry (21st Ed.). Lange Medical Publication. Los Altos, California.
Hemavathi B., Thygaraju K. & Bharathi D., 2002, Effect of thyroxine on the activities of dehydrogenases in silkworm, Bombyx mori L. Indian Journal of Comparative Animal Physiology 20: 59-65.
Herrero-Yraola A., Bakhit S.M.A., Franke P., Weise C., Scheiger M., Jorcke D. & Ziegler M., 2001, Regulation of glutamate dehydrogenase by reversible ADP-ribosylation in mitochondria. European Molecular Biology Organization Journal 20(10): 2404-2412.
Horie Y., 1968, The oxidation of NADPH by the soluble fraction of the fat body of the silkworm, Bombyx mori L. Journal of Insect Physiology 14: 417-424.
Kasmaei F.G. & Mahesha H.B., 2012a, Studies on succinate dehydrogenase and its relationship with economic characters of silkworm, Bombyx mori L. Annals of Biological Research 3(7): 3638-3651.
Kasmaei F.G. & Mahesha H.B., 2012b, Correlation studies on haemolymph and midgut tissue proteins with commercial characters of silkworm, Bombyx mori L. Asian Journal of Experimental Biological Sciences 3(3): 642-653.
Krishnaswami S., 1978, New technology of silkworm rearing. Bulletin No. 2 of Central Sericultural Research & Training Institute (CSRTI), Mysore, India, p. 1-24.
Kumar R. & Gangwar S.K., 2010, Impact of varietal feeding on Samia ricini Donovan in spring and autumn season of Uttar Pradesh. ARPN Journal of Agricultural and Biological Science 5(3): 46-51.
Lee Y.L. & Lardy H.A., 1965, Influence of thyroid hormones on L-glycerophosphate dehydrogenase and other dehydrogenases in various organs of the rat. Journal of Biological Chemistry 240:1427-1436.
Lehninger A.L., 1993, Biochemistry (2nd Ed.). Kalyani Publishers, Ludhiana, New Delhi, India.
Lowry O.H., Rosebrough N.J., Farr A.L. & Randall R.S., 1951, Protein measurement with the Folin Phenol reagent. Journal of Biological Chemistry 93: 265-275.
Maqbool A., 2010 Genetic divergence in some bivoltine silkworm, Bombyx mori L. breeds. PhD Thesis Submitted to Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, p. 1-76.
McCammon M.T., Epstein C.B., Przybyla-Zawislak B., McAlister-Henn L. & Butow R.A., 2003, Global transcription analysis of Krebs tricarboxylic acid cycle mutants reveal an alternating pattern of gene expression and effects on hypoxic and oxidative genes. Molecular Biology of the Cell 14: 958-972.
Nachlas M.M., Morgulis S.P. & Seligman A.M. 1960, A calorimetric method for the determination of Succinate dehydrogenase activity. Journal of Biological Chemistry 235: 499-505.
Naga Jyothi P., Nagalakshmamma K., Suneetha Y. & Sarma P.A., 2009, Enhancement of protein metabolism and enzyme activities in haemolymph of silkworm, Bombyx mori L. by ultrasound treatment during fifth instar development. World Journal of Zoology 4(3): 225-231.
Osanai M., Aigaki T. & Kosuga H., 1987, Arginine degradation cascade as an energy yielding system for sperm maturation in the spermatophore of silkworm, Bombyx mori, [in:] P.P. Mohr (ed.) New Horizons in Sperm Cell Research, p. 185-195.
Pant R. & Jaiswal G., 1981, Photoperiodic effect on transaminase activity, protein and total free amino acid content in the fat body of diapausing pupae of the tasar silkworm, Antheraea mylitta. Indian Journal of Experimental Biology 19: 998-1000.
Raja R. 2000, Appropriate Silkworm Rearing Technology, [in:] H.O. Agrawal, M.K. Seth (eds.) Sericulture in India, p. 289-302. Bishen Singh Mahendra Pal Singh Press, Dehradun, India.
Rajannan K.L., Pattarju H.P., Manjula A.C.A. & Yadav P.R., 1994, Biochemical differences among some selected lines of two different voltine races of Bombyx mori L. Journal of the Entomological Research Society 18(1): 53-60.
Rajasekhar R., 1993, Studies on the temperature induced modulations on the growth and reproductive physiology of silkworm Bombyx mori L., p. 45-48. Ph.D. Thesis, Sri Venkateswara University, Tirupati, India.
Shivkumar G. & Subramanya G., 2015, Quantitative estimation of haemolymph protein during different days of fifth instar larvae in bivoltines, multivoltines and mutants of the silkworm Bombyx mori. Global Journal of Bioscience and Biotechnology 4(3): 239-241.
Sumino T., Masao N. & Masahiko K., 1980, Storage proteins in the silkworm Bombyx mori L. Insect Biochemistry 10: 289-303.
Wani M.Y., Ganie N.A., Mir M.R., Mir S.A., Wani T.A., Yaqoob M. & Murtaza I., 2021, Total protein and total free amino acid estimation in peculiar bivoltine breed of mulberry silkworm (Bombyx mori L.). J. Exp. Zool. India 24(2): 901-908.
Yogananda Murthy V.N., 2015, Estimation of protein concentration in different tissues of popular silkworm (Bombyx mori L.) races. International Journal of Advanced Research 3(1): 254-261.
Yungen M., Lijun J. & Bharathi D., 2002, Impact of Prostaglandin F2 on Silk Quality and Biosynthesis of Silk Proteins of the Silkworm, Bombyx mori L. J. Agric. Urban Entomol. 19(2): 65-71.
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Copyright (c) 2023 Sergiy Yeremenko, Mohd Younus Wani, Nisar Ahmad Ganie, Mushtaq Rasool Mir, Shakeel Ahmad Mir, Munazah Yaqoob, Imtiyaz Murtaza, Tanveer Ahmad Wani, Mohd Farooq Baqual, Oleksii Sukennikov
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