Wastewater treatment by conversion of nitrogen-containing pollution by immobilized microbiocenosis in a biodisk installation
DOI:
https://doi.org/10.12775/EQ.2022.017Keywords
wastewater treatment, nitrogen compounds, immobilized microbiocenosis, deamonization, denitration, nitrification, anammox process, control effects, optimal parametersAbstract
Experimental works are devoted to research the efficiency of deamonization and de-nitrogenation of wastewater during treatment in a biodisk installation. Working hypothesis - immobilization of the microbiocenosis can provide simultaneous deep treatment of wastewater from organic compounds and inorganic nitrogen-containing compounds. The purpose of this work is to assess the efficiency of purification of highly concentrated organic pollutants and mineralized wastewater from nitrogen compounds in a biodisk installation and to determine the main ecological-trophic groups of microorganisms included in the immobilized nitrogen-transforming microbiocenosis. Methods of research of immobilized microbiocenosis - microbiological. physiological, biochemical (inhibitory experiments), natural and model wastewater - hydrochemical. It has been established that in microbiocenosis immobilized on disks conditions that allow heterotrophic and autotrophic microorganisms to actively metabolize organic and inorganic compounds under aerobic and anoxidic conditions are created. The quantitative indicators of the influence on the kinetics of the processes of the concentration of organic substances in the treated wastewater have been determined. On the basis of microbiological, physiological and biochemical studies in the immobilized microbiocenosis, ammonium-acid bacteria (and, possibly, archaea) nitrite-acid and denitrifying bacteria were found, and in the absence of organic substances in the environment - anammox bacteria. Moreover, in the biofilm that was formed in the absence of organic substances in the environment, the activity of anammox bacteria in deamonization significantly exceeded the activity of ammonium oxidizing bacteria and archaea. The obtained results and quantitative requirements were used in the organization of real wastewater treatment in a biodisc plant in industrial conditions.
References
Anyusheva M.G. & Kalyuzhny S.V., 2007, Anaerobic ammonium oxidation: microbiological, chemical and biotechnological aspects. Advances in modern biology. Moscow State University Lomonosov 127(1): 34–43. [in Russian].
Ding S., Zheng P., Lu H., Chen J., Mahmood Q., Abbas G., 2013. Ecological characteristics of anaerobic ammonia oxidizing
bacteria. Appl. Microbiol. Biotechnol. 97: 1841–1849. DOI: 10.1007/s00253-013-4697-0
Feng H., Ding Y., Wang M., Zhou G., Zheng X., He H., Zhang X., Shen D. & Shentu J., 2014, Where are signal molecules likely to be located in anaerobic granular sludge? Water Research 50: 1–9. https://doi.org/10.1016/j.watres.2013.11.021
Gvozdyak P.I. & Sapura O.V., 2009, A simple method of detecting and assessing the intensity of anaerobic processes, which is supervised by the approval of gases. Microbiology and Biotechnology 8: 53–57. [in Ukrainian].
Hassard F., Biddle J., Jeremy R., Cartmell E., Jefferso, B., Tyrrel S. & Stephenson T., 2015, Rotating biological contactors for wastewater treatment – A review. Process Safety and Environmental Protection 94: 285–306. http://dx.doi.org/10.1016/j.psep.2014.07.003
Iurchenko V., Radionov M., Ivanin P. & Melnikova O., 2020, Influence of Deep-Treated Wastewater Discharge on Nitrification Activity in a Natural Reservoirs. J. Ecol. Eng. 21(8): 146–155. https://doi.org/10.12911/22998993/126984 [in Ukrainian].
Ji J., Peng Y., Li X., Zhang Q. & Liu X., 2020, A novel partial nitrification-synchronous anammox and endogenous partial denitrification (PN-SAEPD) process for advanced nitrogen removal from municipal wastewater at ambient temperatures. Water Research 175: 115–690. https://doi.org/10.1016/j.watres.2020.115690
Kallistova A.Yu., Dorofeev A.G., Nikolaev Yu.A. & Kozlov M.N., 2016, The role of anammox bacteria in the purification of waste water from nitrogen compounds. Microbiology 85(2): 126–144. [in Russian].
Lancaster K.M., Caranto J.D., Majer S.H., & Smith M.A., 2018, Alternative Bioenergy: Updates to and Challenges in Nitrification Metalloenzymology. Joule 2(3): 421–441. https://doi.org/10.1016/j.joule.2018.01.018
List of methods for performing measurements (determinations) of the composition and properties of samples of environmental objects, emissions, wastes and discharges provisionally allowed for use by the State Inspectorate of Ukraine, approved by the Head of the State Environmental Inspectorate of Ukraine - Chief State Inspector of Ukraine for Environmental Protection on 01.03.2013 (Accessed: 02.11.2019) [in Ukrainian].
Litty Yu.V., 2012, Anaerobic oxidation of ammonium and methanogenesis in aerobic wastewater treatment systems with immobilization of microorganisms: Diss. PhD. Biol. Sciences: 03.02.03 / 03.01.06 /. Litti Yuriy Vladimirovich, Moscow, 147 pp. [in Russian].
Malyovanyy М., Sakalova, G., Chornomaz N. & Nahurskyy O., 2013, Water sorption purification from ammonium pollution. Chemistry and chemical technology 7(3): 355–358. DOI:10.23939/chcht07.03.355 [in Ukrainian].
Malovanyy А., Plaza E., Trela J. & Malovanyy M., 2014, Combination of ion exchange and partial nitritation/Anammox process for ammonium removal from mainstream municipal wastewater. Water Science & Technology 70(1): 144–151. DOI:10.2166/wst.2014.208 [in Ukrainian].
Malovanyy M., Zhuk V., Sliusar V. & Sereda A., 2018, Two stage treatment of solid waste leachates in aerated lagoons and at municipal wastewater treatment plants. Eastern-European Journal of Enterprise Technologies 1(10): 23–30. DOI:10.15587/1729-4061.2018.122425 [in Ukrainian].
Malovanyy M., Moroz O., Hnatush S., Maslovska O., Zhuk V., Petrushka I., Nykyforov V. & Sereda A., 2019, Perspective Technologies of the Treatment of the Wastewaters with High Content of Organic Pollutants and Ammoniacal Nitrogen. Journal of Ecological Engineering 20(2): 8–15. DOI:10.12911/22998993/94917
Sakalova H., Malovanyy M., Vasylinych T. & Kryklyvyi R., 2019, The Research of Ammonium Concentrations in City Stocks and Further Sedimentation of Ion-Exchange Concentrate. Journal of Ecological Engineering 20(1): 158–164. DOI:10.12911/22998993/93944
Shukla P., 2017, Microbial Biotechnology: An Interdisciplinary Approach. CRC Rress, Rohtak, India, 378 pp.
ter Haseborg E., Zamora T.M., Fröhlich J. & Frimmel F.H., 2010, Nitrifying microorganisms in fixed-bed biofilm reactors fed with different nitrite and ammonia concentrations. Bioresource Technology 101(6): 1701–1706. https://doi.org/10.1016/j.biortech.2009.09.091
Tsytlishvili K., 2020, Method of studying the quality of biological wastewater treatment using complex laboratory equipment: US Pat. 142646 Ukraine: IPC (2006.01) С02F 3/02. No. in 2019 10647; declared 10/28/2019; publ. 25.06.2020, Bull. No. 12 [in Ukrainian].
Tsytlishvili K., 2021, Ecology of immobilized nitrotransforming microbiocenosis in the wastewater treatment systems, diss. Ph.D. Sciences: 101 Environmental Studies, Kharkiv, 186 pp. [in Ukrainian].
Vinogradova A.V. & Kozlova G.A., 2012, Microorganism cultivation. Perm. Nat. researched Polytechnic University, Permian [in Russian].
Wu L., Shen M., Li J., Huang S., Li Z., Yan Z. & Peng Y., 2019, Cooperation between partial-nitrification, complete ammonia oxidation (comammox), and anaerobic ammonia oxidation (anammox) in sludge digestion liquid for nitrogen removal. Environmental Pollution 254(Pt A): 112965. https://doi.org/10.1016/j.envpol.2019.112965
Wu Y., Guo Y., Lin X., Zhong W. & Jia Z., 2012, Inhibition of bacterial ammonia oxidation by organohydrazines in soil microcosms. Front Microbiol. 3(10). DOI: 10.3389/fmicb.2012.00010
Xiang Y., Shao Z., Chai H., Ji F. & He Q., 2020, Functional microorganisms and enzymes related nitrogen cycle in the biofilm performing simultaneous nitrification and denitrification. Bioresource Technology 314: 123697. https://doi.org/10.1016/j.biortech.2020.123697
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Stats
Number of views and downloads: 1179
Number of citations: 2
