Effects of phenolic compounds on the sulfidogenic activity of Desulfotomaculum sp. and Desulfovibrio desulfuricans bacteria
DOI:
https://doi.org/10.12775/EQ.2021.039Keywords
pyrogallol, hydroquinone, sulfate ion, bioremediation, hydrogen sulfide, catecholAbstract
Purification of environment from the pollutants of organic and inorganic matter is one of the actual problem of nowadays. Compounds with benzene ring are among the most hazardous pollutants. The danger of getting the phenolic compounds into the environment is connected with their toxicity for biological objects and stability. One of the most effective methods of environment detoxification is the usage of microorganisms. The aim of this work was to determine the ability of Desulfotomaculum sp. AR1 and Desulfovibrio desulfuricans Ya-11 bacteria to utilize phenol derivatives as the carbon source and to study the effects of phenolic compounds on the sulfidogenic activity of these bacteria. Both bacterial strains grew in the media with pyrogallol and hydroquinone, but the biomass in the medium with hydroquinone was considerably lower. Inhibition of sulfate ion utilization by bacteria was found during the growth of Desulfotomaculum sp. AR1 and D. desulfuricans Ya-11 in the medium with phenolic compounds. Effectiveness of pyrogallol utilization by both bacterial strains was for 4 times higher, compared to the effectiveness of hydroquinone utilization. Desulfotomaculum sp. AR1 and D. desulfuricans Ya-11 bacteria are also capable for growth in the medium with sodium benzoate, utilizing this aromatic compound as carbon and energy source. Process of catechol degradation by Desulfotomaculum sp. AR1 bacteria under anaerobic conditions is ineffective.
Desulfotomaculum sp. AR1 and Desulfovibrio desulfuricans Ya-11 bacteria are capable for growth in the medium with phenolic compounds, utilizing them as the sole carbon source and removing them from the environment. ANOVA showed significant differences between the amount of utilized SO42- in the control medium and medium with aromatic compounds. Presence of aromatic compounds in the medium inhibits the sulfate reduction in Desulfotomaculum sp. AR1 and D. desulfuricans Ya-11 bacteria, which results in the decrease of toxic hydrogen sulfide accumulation in the medium.
Usage of microorganisms with the aim of environment detoxification is a promising way of water biotopes remediation technologies.
References
Auburger G. & Winter J., 1992, Purification and characterization of benzoyl-CoA ligase from a syntrophic, benzoate-degrading, anaerobic mixed. Applied Microbiology and Biotechnology 37: 789–795. DOI: 10.1007/BF00174847
Baltser D.V. & Pavlovich L.B., 2012, Ispolzovanie ochischennyh fenolnyh stochnyh vod v vodosnabzhenii koksohimicheskogo proizvodstva [The use of treated phenolic industrial effluents for process water supply of coking plants]. Water supply and sanitary technique 12: 52–58.
Bargiel P. & Zabochnicka-Swiatek M., 2018, Technologies of Coke Wastewater Treatment in the Frame of Legislation in Force. Ochrona Srodowiska i Zasobоw Naturalnych 29(1): 11–15.
Cueva C., Moreno-Arribas M.V., Martіn-Alvarez P.J., Bills G., Vicente M.F., Basilio A. et al., 2010, Antimicrobial activity of phenolic acids against commensal, probiotic and pathogenic bacteria. Research in Microbiology 161(5): 372–382. DOI: 10.1016/j.resmic.2010.04.006
Dalal S., Pandey R.R. & Dubey R.C., 2012, Bacterial Degradation of Phenol and Cyanide from Industrial Wastewater, [іn:] D.K. Maheshwari, R.C. Dubey (еds.), Bioremedial of Pollutants, IK International Pub. Com, New Delhi, India, p. 1–23.
Demadis K., Mavredaki E., Stathoulopoulou A., Neofotistou E. & Mantzaridis C., 2007, Industrial water systems: problems, challenges and solutions for the process industries. Desalination 213(1–3): 38–46. DOI: 10.1016/j.desal.2006.01.042
Dudnik S.V. & Yevtushenko M.Yu., 2013, Water toxicology: basic theoretical positions and their practical application. K.: View of the Ukrainian Phytosociological Center.
Galkina О. & Dehtiar M., 2019, Tekhnolohii ochyshchennia fenolnykh stichnykh vod [Phenolic wastewater treatment technologies]. Ecological Sciences 1(24): 32–36. DOI: 10.32846/2306-9716-2019-1-24-2-7
Gibson J. & Harwood C.S., 2002, Мetabolic diversity in aromatic compound utilization by anaerobic microbes. Annual Review of Microbiology 56: 345–369.
Gorny N. & Schink B., 1994, Anaerobic degradation of catechol by Desulfobacterium sp. strain Cat2 proceeds via carboxylation to protocatechuate. Applied and Environmental Microbiology 60: 3396–3340.
Haidin A.M. & Zozulia I.I., 2009, New lakes of Lviv region, 2nd ed. Afisha, Lviv.
Khomenkov V.G., Shevelev A.B., Zhukov V.G., Zagustina N.A., Bezborodov A.M. & Popov V.O., 2008, Orhanyzatsyia metabolycheskykh putei y molekuliarno-henetycheskye mekhanyzmy byoderhadatsyy ksenobyotykov u mykroorhanyzmov [Organization of metabolic pathways and molecular-genetic mechanisms of xenobiotic degradation in microorganisms]. Applied Biochemistry and Microbiology 44(2): 133–152.
Kopcha N., Sadlyak A. & Bokshan O., 2010, Fenoloksydazna aktyvnist bakterii rodiv Pseudomonas ta Klebsiella, yikh zdatnist do detoksykatsii fenolu [Рhenoloxydase activity of bacteria Pseudomonas and Klebsiella genes, their ability to degradation of phenol]. Scientific Bulletin of the Uzhgorod University, Series Biology 28: 19–22.
Lastukhin Yu.O. & Voronov S.A., 2006, Organic chemistry. Lviv: Tsentr Yevropy.
Oliferchuk V.P., Matvienko M.T. & Voуtovich I.G., 2009, Mozhlyvist vykorystannia osadu stichnykh vod ochysnykh sporud Lvova dlia vyrobnytstva biohazu [Flow waters sediment use posibilities of Lviv's sewage disposal plant for biogas production]. Scientific Bulletin of NUFWT of Ukraine 19(9): 72–76.
Peretyatko T.B., Hnatush S.O. & Gudz S.P., 2006, Sulfatvidnovliuvalni bakterii Yavorivskoho sirkovoho rodovyshcha [Sulfate-reducing bacteria from Yavoriv storage lake]. Mikrobiolohichnyi Zhurnal 68(5): 84–91.
Postgate J.R., 1984, The sulfate-reducing bacteria. 2nd ed. Cambridge: Cambridge University Press.
Schink B., Philipp B. & Müller J., 2000, Anaerobic Degradation of Phenolic Compounds. Naturwissenschaften 87(1): 12–23. DOI: 10.1007/s001140050002
Sholyak K.V., Peretyatko T.B. & Gudz S.P., 2013, Poshyrennia khromrezystentnykh mikroorhanizmiv u stichnykh vodakh [Distribution of chromium-resistant microorganisms in sewage]. Agroecological Journal 3: 81–85.
Shved O.M., Vydrynska O.K., Chervetsova V.H., Hubrii Z.V. & Novikov V.P., 2012, Novi pidkhody do biolohichnoho ochyshchennia stichnykh vod mista Lvova [New approaches to biological wastewater treatment in Lviv]. Bulletin of the National University "Lviv Polytechnic" series: "Chemistry, technology of substances and their applications" 726: 145–152.
Siddique S., Parveen Z., Ali Z. & Zaheer М., 2012, Qualitative and Quantitative Estimation of Hydroquinone in Skin Whitening Cosmetics. Journal of Cosmetics, Dermatological Sciences and Applications 2: 224-228. DOI: 10.4236/jcdsa.2012.23042
Sugiyama M., 2002, Incassignee. Reagent composition for measuring hydrogen sulfide and method for measuring hydrogen sulfide. United States Patent 6,340,596 B1.
Taras U.M., 2013, Problemy rekultyvatsii sirchanoho karieru v zoni diialnosti Yavorivskoho derzhavnoho hirnycho-khimichnoho pidpryiemsta "Sirka" [The revegetation problems of sulphuric pit during activity of the Yavoriv state mines-chemical enterprise]. Scientific Bulletin of UNFU 23(2): 154–158.
Verkholiak N.S. & Peretyatko T.B., 2018, Morfofiziolohichni vlastyvosti sulfatvidnovliuvalnykh bakterii, vydilenykh iz systemy ochyshchennia stichnykh vod m. Lvova [Morphophysiological properties of sulfate-reducing baсteria isolated from the system of Lviv wastewater treatment]. Microbіology and biotechnology 4: 19–29. DOI: 10.18524/2307-4663.2018.4(44).139440
Voloshyn M.D., Shcherbak O.L., Chernenko Ya.M. & Korniienko I.M., 2009, Improving the technology of biological wastewater treatment. Dniprodzerzhynskyi Derzhavnyi Tekhnichnyi Universytet, Dniprodzerzhynsk.
Zhang L.-L., Wang Y.-M., Xu M. & Chen J.-H., 2015, Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method. Biomass Chemical Engineering 49(2): 12–16.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Natalіa Verkholiak, Taras Peretyatko, Andrii Halushka
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Stats
Number of views and downloads: 391
Number of citations: 0
