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Quality in Sport

Cortisol as a Blood Biomarker for Monitoring Endurance Training - A Narrative Review
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  • Cortisol as a Blood Biomarker for Monitoring Endurance Training - A Narrative Review
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Cortisol as a Blood Biomarker for Monitoring Endurance Training - A Narrative Review

Authors

  • Luiza Stadnik St. Adalbert's Hospital Copernicus PL Sp. z o.o., Gdańsk, Poland https://orcid.org/0009-0009-6854-4905
  • Alicja Kozłowska The PCK Maritime Hospital in Gdynia https://orcid.org/0009-0000-5991-7401
  • Karolina Przybysz The PCK Maritime Hospital in Gdynia https://orcid.org/0009-0002-9001-829X
  • Natalia Woroniecka Independent Public Healthcare Facility of the Ministry of Interior and Administration, Gdańsk, Poland https://orcid.org/0009-0006-4713-4526
  • Adela Dzwonkowska Tczew Hospitals S.A., Tczew, Poland https://orcid.org/0009-0004-7026-7437
  • Natalia Zienkiewicz The PCK Maritime Hospital in Gdynia https://orcid.org/0009-0009-3380-0470
  • Daria Trocka St. Adalbert's Hospital Copernicus PL Sp. z o.o., Gdańsk, Poland https://orcid.org/0000-0001-9684-5881
  • Jarosław Rachoń 7th Naval Hospital, Gdańsk, Poland https://orcid.org/0009-0007-9691-2221
  • Nicol Szerenos https://orcid.org/0009-0009-2754-8344 https://orcid.org/0009-0009-2754-8344
  • Jakub Dzwonkowski Nicolas Copernicus Provincial Hospital, Koszalin, Poland https://orcid.org/0009-0006-5087-0698

DOI:

https://doi.org/10.12775/QS.2026.65.74045

Keywords

cortisol, endurance training, exercise physiology, training monitoring, training load, physiological stress, overtraining syndrome, athlete monitoring, Hypothalamic-Pituitary- Adrenal Axis

Abstract

Background.

Blood cortisol is a key biomarker of hypothalamic-pituitary-adrenal (HPA) axis activity and is widely used in sports science to assess physiological and psychological stress.
However, its interpretation is limited by considerable biological variability and multiple influencing factors.

Aim.

To summarize current evidence on the physiological role of blood cortisol in endurance exercise and its usefulness for monitoring training load, recovery, and adaptation.

Material and methods.

A narrative review of peer-reviewed studies, systematic reviews,
meta-analyses, and guidelines on cortisol physiology, exercise endocrinology, and athlete monitoring was conducted.

Results.

Blood cortisol reflects the physiological stress response and supports metabolic and
immune regulation during endurance exercise. Acute increases are a normal adaptive response, whereas persistent alterations may indicate excessive training stress when interpreted in context. Due to circadian variation and other confounding factors, single measurements have limited value. Current evidence favors longitudinal within-athlete monitoring combined with physiological, psychometric, hormonal, and performance measures.
Conclusions.

Blood cortisol is a useful component of endurance athlete monitoring but
should not be used as a stand-alone marker. Its greatest value lies in repeated standardized assessments interpreted within a multimodal monitoring approach.

Key words:

cortisol, endurance training, exercise physiology, training monitoring, training
load, physiological stress, overtraining syndrome, athlete monitoring, Hypothalamic-Pituitary-Adrenal Axis

References

1. Meeusen R, Duclos M, Foster C, et al. Prevention, diagnosis, and treatment of the

overtraining syndrome: joint consensus statement of the European College of Sport

Science and the American College of Sports Medicine. Med Sci Sports Exerc.

2013;45(1):186-205. https://doi.org/10.1249/MSS.0b013e318279a10a

2. Halson SL. Monitoring training load to understand fatigue in athletes. Sports Med.

2014;44 Suppl 2(Suppl 2):S139-S147.https://doi.org/10.1007/s40279-014-0253-z

3. Lee EC, Fragala MS, Kavouras SA, Queen RM, Pryor JL, Casa DJ. Biomarkers in

Sports and Exercise: Tracking Health, Performance, and Recovery in Athletes. J

Strength Cond Res. 2017;31(10):2920-2937.

https://doi.org/10.1519/JSC.0000000000002122

4. Soler-López A, Moreno-Villanueva A, Gómez-Carmona CD, Pino-Ortega J. The Role

of Biomarkers in Monitoring Chronic Fatigue Among Male Professional Team

Athletes: A Systematic Review. Sensors. 2024; 24(21):6862.

https://doi.org/10.3390/s24216862

5. Hackney AC, Walz EA. Hormonal adaptation and the stress of exercise training: the

role of glucocorticoids. Trends Sport Sci. 2013;20(4):165-171.

https://pmc.ncbi.nlm.nih.gov/articles/PMC5988244/

6. Popovic B, Popovic D, Macut D, et al. Acute Response to Endurance Exercise Stress:

Focus on Catabolic/anabolic Interplay Between Cortisol, Testosterone, and Sex

Hormone Binding Globulin in Professional Athletes. J Med Biochem. 2019;38(1):6-

12. Published 2019 Mar 1. https://doi.org/10.2478/jomb-2018-0016

7. Anderson T, Wideman L. Exercise and the Cortisol Awakening Response: A

Systematic Review. Sports Med Open. 2017;3(1):37. Published 2017 Oct 10.

https://doi.org/10.1186/s40798-017-0102-3

8. Wright KD, Hickman R, Laudenslager ML. Hair Cortisol Analysis: A Promising

Biomarker of HPA Activation in Older Adults. Gerontologist. 2015;55 Suppl 1(Suppl

1):S140-S145. https://doi.org/10.1093/geront/gnu174

9. Choi MH. Clinical and Technical Aspects in Free Cortisol Measurement. Endocrinol

Metab (Seoul). 2022;37(4):599-607. https://doi.org/10.3803/EnM.2022.1549

10. Herman JP, McKlveen JM, Ghosal S, et al. Regulation of the Hypothalamic-Pituitary-

Adrenocortical Stress Response. Compr Physiol. 2016;6(2):603-621. Published 2016

Mar 15. https://doi.org/10.1002/cphy.c150015

11. Dumbell R, Matveeva O, Oster H. Circadian Clocks, Stress, and Immunity. Front

Endocrinol (Lausanne). 2016;7:37. Published 2016 May 2.

https://doi.org/10.3389/fendo.2016.00037

12. Hill EE, Zack E, Battaglini C, Viru M, Viru A, Hackney AC. Exercise and circulating

cortisol levels: the intensity threshold effect. J Endocrinol Invest. 2008 Jul;31(7):587-

91. https://doi.org/10.1007/BF03345606

13. Nicolaides NC, Charmandari E, Kino T, Chrousos GP. Stress-Related and Circadian

Secretion and Target Tissue Actions of Glucocorticoids: Impact on Health. Front

Endocrinol (Lausanne). 2017 Apr 28;8:70. https://doi.org/10.3389/fendo.2017.00070

14. Sapolsky RM, Romero LM, Munck AU. How do glucocorticoids influence stress

responses? Integrating permissive, suppressive, stimulatory, and preparative actions.

Endocr Rev. 2000 Feb;21(1):55-89. https://doi.org/10.1210/edrv.21.1.0389

15. Cain DW, Cidlowski JA. Immune regulation by glucocorticoids. Nat Rev Immunol.

2017;17(4):233-247. https://doi.org/10.1038/nri.2017.1

16. Nieman DC, Wentz LM. The compelling link between physical activity and the body's

defense system. J Sport Health Sci. 2019;8(3):201-217.

https://doi.org/10.1016/j.jshs.2018.09.009

17. Gleeson M. Immune function in sport and exercise. J Appl Physiol (1985).

2007;103(2):693-699. https://doi.org/10.1152/japplphysiol.00008.2007

18. Walsh NP. Recommendations to maintain immune health in athletes. Eur J Sport Sci.

2018;18(6):820-831. https://doi.org/10.1080/17461391.2018.1449895

19. Duclos M, Tabarin A. Exercise and the Hypothalamo-Pituitary-Adrenal Axis. Front

Horm Res. 2016;47:12-26. https://doi.org/10.1159/000445149

20. Dote-Montero M, Carneiro-Barrera A, Martinez-Vizcaino V, Ruiz JR, Amaro-Gahete

FJ. Acute effect of HIIT on testosterone and cortisol levels in healthy individuals: A

systematic review and meta-analysis. Scand J Med Sci Sports. 2021;31(9):1722-1744.

https://doi.org/10.1111/sms.13999

21. Peake JM, Tan SJ, Markworth JF, et al. Metabolic and hormonal responses to

isoenergetic high-intensity interval exercise and continuous moderate-intensity

exercise. Am J Physiol Endocrinol Metab. 2015;308(7):E539-E552.

https://doi.org/10.1152/ajpendo.00276.2014

22. VanBruggen MD, Hackney AC, McMurray RG, Ondrak KS. The relationship between

serum and salivary cortisol levels in response to different intensities of exercise. Int J

Sports Physiol Perform. 2011;6(3):396-407. https://doi.org/10.1123/ijspp.6.3.396

23. Viru A, Karelson K, Smirnova T. Stability and variability in hormonal responses to

prolonged exercise. Int J Sports Med. 1992;13(3):230-235. https://doi.org/10.1055/s-

2007-1021259

24. Tsigos C, Chrousos GP. Hypothalamic-pituitary-adrenal axis, neuroendocrine factors

and stress. J Psychosom Res. 2002;53(4):865-871. https://doi.org/10.1016/S0022-

3999(02)00429-4

25. Leproult R, Van Cauter E. Role of sleep and sleep loss in hormonal release and

metabolism. Endocr Dev. 2010;17:11-21. https://doi.org/10.1159/000262524

26. Kraemer WJ, Ratamess NA. Hormonal responses and adaptations to exercise and

training. Sports Med. 2005;35(4):339-361. https://doi.org/10.2165/00007256-

200535040-00004

27. Logan-Sprenger HM, Heigenhauser GJF, Killian KJ, Spriet LL. Muscle metabolism

during exercise with dehydration. J Appl Physiol. 2012;112(12):1966-1974.

https://doi.org/10.1152/japplphysiol.00062.2012

28. Kajantie E, Phillips DIW. The effects of sex and hormonal status on the physiological

response to acute psychosocial stress. Psychoneuroendocrinology. 2006;31(2):151-

178. https://doi.org/10.1016/j.psyneuen.2005.07.002

29. Gatti R, De Palo EF. An update: salivary hormones and physical exercise. Scand J

Med Sci Sports. 2011;21(2):157-169. https://doi.org/10.1111/j.1600-

0838.2010.01252.x

30. Kirschbaum C, Hellhammer DH. Salivary cortisol in psychoneuroendocrine research.

Psychoneuroendocrinology. 1994;19(4):313-333. https://doi.org/10.1016/0306-

4530(94)90013-2

31. Vining RF, McGinley RA, Maksvytis JJ, Ho KY. Salivary cortisol: a better measure

of adrenal cortical function than serum cortisol. Clin Chem. 1983;29(10):1850-1853.

https://doi.org/10.1177/000456328302000601

32. Russell E, Koren G, Rieder M, et al. Hair cortisol as a biological marker of chronic

stress. Curr Opin Psychiatry. 2012;25(1):63-69.

https://doi.org/10.1097/YCO.0b013e32834c87ea

33. Wood P. Steroid assays-why do they vary so much? J Endocrinol. 2013;216(1):T1-

T10. https://doi.org/10.1530/JOE-12-0449

34. Raff H. Utility of salivary cortisol measurements in Cushing’s syndrome and adrenal

insufficiency. J Clin Endocrinol Metab. 2009;94(10):3647-3655.

https://doi.org/10.1210/jc.2009-1166

35. Taylor RL, Machacek D, Singh RJ. Validation of a high-throughput liquid

chromatography-tandem mass spectrometry method for urinary cortisol and cortisone.

Clin Chem. 2002;48(9):1511-1519.

https://doi.org/10.1093/clinchem/48.9.1511

36. Hackney AC. Stress and the neuroendocrine system: the role of exercise as a stressor

and modifier of stress. Expert Rev Endocrinol Metab. 2006;1(6):783-792.

https://doi.org/10.1586/17446651.1.6.783

37. Athanasiou N, Bogdanis GC, Mastorakos G. Endocrine responses of the stress system

to different types of exercise. Rev Endocr Metab Disord. 2023;24(2):251-266.

https://doi.org/doi:10.1007/s11154-022-09758-1

38. Saw AE, Main LC, Gastin PB. Monitoring the athlete training response: subjective

self-reported measures trump commonly used objective measures. Br J Sports Med.

2016;50(5):281-291. https://doi.org/10.1136/bjsports-2015-094758

39. Cadegiani FA, Kater CE. Hormonal aspects of overtraining syndrome: a systematic

review. BMC Sports Sci Med Rehabil. 2017;9:14. https://doi.org/10.1186/s13102-017-

0079-8

40. Kreher JB, Schwartz JB. Overtraining syndrome: a practical guide. Sports Health.

2012;4(2):128-138. https://doi.org/10.1177/1941738111434406

41. Rebelo A, Bishop C, Thorpe RT, Turner AN, Gabbett TJ. Monitoring Training Effects

in Athletes: A Multidimensional Framework for Decision-Making. Sports Med. 2026

Mar 13. https://doi.org/10.1007/s40279-026-02417-4

42. Mondal S, Hathi DK, Bhattacharya S, Kalra S. The Testosterone: Cortisol Ratio - A

Tool with Practical Use and Research Potential in Endocrinology. Indian J Endocrinol

Metab. 2025;29(5):510-516. https://doi.org/10.4103/ijem.ijem_85_25

43. Pedlar CR, Newell J, Lewis NA. Blood Biomarker Profiling and Monitoring for High-

Performance Physiology and Nutrition: Current Perspectives, Limitations and

Recommendations. Sports Med. 2019 Dec;49(Suppl 2):185-198.

https://doi.org/10.1007/s40279-019-01158-x

44. Souza RA, Beltran OAB, Zapata DM, Silva E, Freitas WZ, Junior RV, da Silva FF,

Higino WP. Heart rate variability, salivary cortisol and competitive state anxiety

responses during pre-competition and pre-training moments. Biol Sport. 2019

Mar;36(1):39-46. https://doi.org/10.5114/biolsport.2018.78905

45. Mănescu DC, Plăstoi CD, Pîrvan A, Pașcan CD, Păun L, Sersea IE, Niculescu B,

Popescu VE, Voinea A, Popescu A. Biomarkers as Temporal Signals: A Decision-

Linked Multi-Layer Framework for Exercise Recovery, Overload, and Adaptation. Int

J Mol Sci. 2026; 27(8):3675. https://doi.org/10.3390/ijms27083675

46. Urhausen A, Kindermann W. Diagnosis of overtraining: what tools do we have?

Sports Med. 2002;32(2):95-102. https://doi.org/10.2165/00007256-200232020-00002

47. Hecksteden A, Skorski S, Schwindling S, Hammes D, Pfeiffer M, Kellmann M,

Ferrauti A, Meyer T. Blood-Borne Markers of Fatigue in Competitive Athletes -

Results from Simulated Training Camps. PLoS One. 2016 Feb 18;11(2):e0148810.

https://doi.org/10.1371/journal.pone.0148810

48. Armstrong LE, Bergeron MF, Lee EC, Mershon JE, Armstrong EM. Overtraining

Syndrome as a Complex Systems Phenomenon. Front Netw Physiol. 2022 Jan

18;1:794392. https://doi.org/10.3389/fnetp.2021.794392

49. Vignesh V, Castro-Dominguez B, James TD, Gamble-Turner JM, Lightman S, Reis

NM. Advancements in Cortisol Detection: From Conventional Methods to Next-

Generation Technologies for Enhanced Hormone Monitoring. ACS Sens. 2024 Apr

26;9(4):1666-1681. https://doi.org/10.1021/acssensors.3c01912

50. Dunbar J, Hazell G, Jehanli A. Evaluation of a new point of care quantitative cube

reader for salivary analysis in the English Premier League soccer environment. Br J

Sports Med. 2015;49(suppl 2):A2-A3. https://doi.org/10.1136/bjsports-2015-

095576.7

51. Assalve G, Lunetti P, Di Cagno A, De Luca EW, Aldegheri S, Zara V, Ferramosca A.

Advanced Wearable Devices for Monitoring Sweat Biochemical Markers in Athletic

Performance: A Comprehensive Review. Biosensors (Basel). 2024 Nov

26;14(12):574. doi: 10.3390/bios14120574 https://doi.org/10.3390/bios14120574

52. Rist B, Pearce AJ. Tiered Levels of Resting Cortisol in an Athletic Population. A

Potential Role for Interpretation in Biopsychosocial Assessment? J Funct Morphol

Kinesiol. 2019 Jan 21;4(1):8. https://doi.org/10.3390/jfmk4010008

53. Minetto MA, Lanfranco F, Tibaudi A, Baldi M, Termine A, Ghigo E. Changes in

awakening cortisol response and midnight salivary cortisol are sensitive markers of

strenuous training-induced fatigue. J Endocrinol Invest. 2008 Jan;31(1):16-24.

https://doi.org/10.1007/BF03345561

54. Kallen VL, Stubbe JH, Zwolle HJ, Valk P. Capturing effort and recovery: reactive and

recuperative cortisol responses to competition in well-trained rowers. BMJ Open Sport

Exerc Med. 2017 Jul 6;3(1):e000235. https://doi.org/10.1136/bmjsem-2017-000235

55. Nuuttila OP, Nummela A, Korhonen E, Häkkinen K, Kyröläinen H. Individualized

Endurance Training Based on Recovery and Training Status in Recreational Runners.

Med Sci Sports Exerc. 2022 Oct 1;54(10):1690-1701.

https://doi.org/10.1249/MSS.0000000000002968

56. Bourdon PC, Cardinale M, Murray A, et al. Monitoring Athlete Training Loads:

Consensus Statement. Int J Sports Physiol Perform. 2017;12(s2):S2-161.

https://doi.org/10.1123/ijspp.2017-0208

57. Hecksteden A, Kraushaar J, Scharhag-Rosenberger F, Theisen D, Senn S, Meyer T.

Individual response to exercise training - a statistical perspective. J Appl Physiol.

2015;118(12):1450-1459. https://doi.org/10.1152/japplphysiol.00714.2014

58. Hacker S, Keck J, Reichel T, Eder K, Ringseis R, Krüger K, Krüger B. Biomarkers in

Endurance Exercise: Individualized Regulation and Predictive Value. Transl Sports

Med. 2023 Dec 14;2023:6614990. https://doi.org/10.1155/2023/6614990

59. Claudino JG, Capanema DO, de Souza TV, Serrão JC, Machado Pereira AC, Nassis

GP. Current Approaches to the Use of Artificial Intelligence for Injury Risk

Assessment and Performance Prediction in Team Sports: a Systematic Review. Sports

Med Open. 2019 Jul 3;5(1):28. https://doi.org/10.1186/s40798-019-0202-3

60. Van Eetvelde H, Mendonça LD, Ley C, Seil R, Tischer T. Machine learning methods

in sport injury prediction and prevention: a systematic review. J Exp Orthop. 2021 Apr

14;8(1):27. https://doi.org/10.1186/s40634-021-00346-x

61. Haller N, Behringer M, Reichel T, Wahl P, Simon P, Krüger K, Zimmer P, Stöggl T.

Blood-Based Biomarkers for Managing Workload in Athletes: Considerations and

Recommendations for Evidence-Based Use of Established Biomarkers. Sports Med.

2023 Jul;53(7):1315-1333. https://doi.org/10.1007/s40279-023-01836-x

Quality in Sport

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2026-08-03

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STADNIK, Luiza, KOZŁOWSKA, Alicja, PRZYBYSZ, Karolina, WORONIECKA, Natalia, DZWONKOWSKA, Adela, ZIENKIEWICZ, Natalia, TROCKA, Daria, RACHOŃ, Jarosław, SZERENOS, Nicol and DZWONKOWSKI, Jakub. Cortisol as a Blood Biomarker for Monitoring Endurance Training - A Narrative Review. Quality in Sport. Online. 3 August 2026. Vol. 65, p. 74045. [Accessed 9 August 2026]. DOI 10.12775/QS.2026.65.74045.
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Copyright (c) 2026 Luiza Stadnik, Alicja Kozłowska, Karolina Przybysz, Natalia Woroniecka, Adela Dzwonkowska, Natalia Zienkiewicz, Daria Trocka, Jarosław Rachoń, Nicol Szerenos, Jakub Dzwonkowski

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