Morphofunctional state of the lungs respiratory part in normotensive and hypertensive rats after combined exposure to intermittent hypoxia and melatonin

Roman Yanko, Mikhail Levashov, Elena Chaka, Sergey Safonov

DOI: http://dx.doi.org/10.12775/JEHS.2021.11.01.006

Abstract


Aim. The purpose of this work was to study and compare the combined effect of intermittent normobaric hypoxia (INH) and melatonin on the morphological and biochemical indices of the lungs respiratory part in Wistar and spontaneous hypertensive rats (SHR).

Material and methods. The studies were conducted on 48 young male rats Wistar and SHR lines. The experimental rats were daily exposed to hypoxic gas mixture (12 % oxygen in nitrogen) in intermittent mode: 15 min deoxygenation / 15 min reoxygenation for 2 hours. Animals also received oral melatonin at a dose of 5 mg /1 kg of body weight once daily.

Results. The sizes of alveolus were reduced, but their number and placement density were increased in SHR rats after a combined effect of INH and melatonin. The results showed a significant decrease of the interalveolar septum thickness and a tendency to decrease of the hydroxyproline concentration in the lung tissue of the experimental rats, regardless of their line.

Conclusions. The 28-day combined effect of INH and melatonin increase the total alveolar surface area and reduce the amount of connective tissue in the lungs of experimental rats. These changes can improve the efficiency of the intrapulmonary gas exchange processes.


Keywords


intermittent normobaric hypoxia; melatonin; lungs

Full Text:

PDF

References


Poulter NR, Prabhakaran D, Caulfield M. Hypertension. Lancet. 2015; 386(9995):801-812. doi: 10.1016/S0140-6736(14)61468-61469.

Imaizumi Y, Eguchi K, Kario K. Lung disease and hypertension. Pulse (Basel). 2015; 2(1-4): 103-112. doi:10.1159/000381684.

Yanko RV, Berezovskij VA, Levashov MI. Vlijanie preryvistoj gipoksii na morfofunkcional'noe sostojanie shhitovidnoj zhelezy i pecheni [Influence of intermittent hypoxia on the morphofunctional state of the thyroid and liver]. Ross. fiziol. zhurn. im. M.I. Sechenova, 2017; 103(5):553-561. (in Russian).

Navarrete-Opazo A, Mitchell GS. Therapeutic potential of intermittent hypoxia: a matter of dose. Am J Physiol Regul Integr Comp Physiol. 2014; 307(10):1181-1197. doi:10.1152/ajpregu.00208.2014.

Tordjman S, Chokron S, Delorme R, et al. Melatonin: pharmacology, functions and therapeutic benefits. Curr Neuropharmacol. 2017; 15(3):434-443. doi: 10.2174/1570159X14666161228122115.

Taslidere E, Esrefoglu М, Elbe Н, et al. Protective effects of melatonin and quercetin on experimental lung injury induced by carbon tetrachloride in rats. Exp Lung Res. 2014; 40:59-65. doi: 10.3109/01902148.2013.866181.

Yanko R, Levashov M, Chaka E, et al. Effect of intermittent normobaric hypoxia on the morphological changes in the respiratory part of lungs in different seasons of the year. Journal of Education, Health and Sport. 2018; 8(5):244-252. doi:10.5281/zenodo.1262716.

Korzhevskij DJe, Giljarov AV. Osnovy gistologicheskoj tehniki [Basics of histological technique], SPb.: SpecLit; 2010. (in Russian).

Vejbel' JeR. Morfometrija legkih cheloveka [Morphometry of the human lungs], M.: Medicina; 1970. (in Russian).

Li P, Wu G. Roles of dietary glycine, proline, and hydroxyproline in collagen synthesis and animal growth. Amino Acids. 2018; 50(1):29-38. doi: 10.1007/s00726-017-2490-6.

Simonjan LG. Lipidy i ih rol' v razvitii legochnyh zabolevanij [Lipids and their role in the development of pulmonary diseases]. Medicinskaja nauka Armenii NAN RA, 2013; LIII(4):66-72. (in Russian).

Neves RVP, Souza MK, Passos CS, et al. Resistance training in spontaneously hypertensive rats with severe hypertension. Arq Bras Cardiol. 2016; 106(3):201-209. doi: 10.5935/abc.20160019.

Knudsen L, Ochs M. The micromechanics of lung alveoli: structure and function of surfactant and tissue components. Histochem Cell Biol. 2018; 150(6):661-676. doi: 10.1007/s00418-018-1747-9.

Karimfar M, Rostami S, Haghani К, et al. Melatonin alleviates bleomycin-induced pulmonary fibrosis in mice. Biol Regul Homeost Agents. 2015; 29:327-334.

Yildirim Z, Kotuk M, Erdogan H, et al. Preventive effect of melatonin on bleomycin-induced lung fibrosis in rats. J Pineal Res. 2006; 40(1):27-33. doi: 10.1111/j.1600-079X.2005.00272.x.

Zhou L, Qian ZX, Li F, et al. The effect of melatonin on the regulation of collagen accumulation and matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 mRNA and protein in a murine model of chronic asthma. Zhonghua Jie He He Hu Xi Za Zhi. 2007; 30(7):527–532.

Kuruş M, Eşrefoğlu М, Otlu А. An experimental study of the histopathological effects of melatonin on cyclosporin induced lung damage. Turkish Thoracic J. 2008; 8(1):1-6.

Wei H, Zhiqiang М, Shuai J, et al. Melatonin: the dawning of a treatment for fibrosis? J Pineal Res. 2016; 60(2):121-131. doi: 10.1111/jpi.12302.

Estrada KD, Chesler NC. Collagen-related gene and protein expression changes in the lung in response to chronic hypoxia. Biomech Model Mechanobiol. 2009; 8(4):263-272. doi: 10.1007/s10237-008-0133-2.

Falanga V, Zhou L, Yufit T. Low oxygen tension stimulates collagen synthesis and COL1A1 transcription through the action of TGF-beta1. J Cell Physiol. 2002; 191(1):42-50. doi: 10.1002/jcp.10065.

Yanko RV, Berezovskiy VA, Chaka OG, et al. Stan respiratornoho viddilu lehenʹ hipertenzyvnykh ta normotenzyvnykh shchuriv pislya vplyvu melatoninu [State of the respiratory part of the lung of hypertensive and normotensive rats after melatonin exposure]. Ukrayinsʹkyy pulʹmonolohichnyy zhurnal. 2017; 4:68-71. (in Ukrainian).

Shin IS, Park JW, Shin NR, et al. Melatonin reduces airway inflammation in ovalbumin-induced asthma. Immunobiology. 2014;(10):138-147. doi: 10.1016/j.imbio.2014.08.004.

Suleymanoglu S, Cekmez F, Cetinkaya M, et al. Protective effects of melatonin therapy in model for neonatal hyperoxic lung injury. Altern Ther Health Med. 2014; 20(5):24-29.

Farías JG, Zepeda AB, Calaf GM. Melatonin protects the heart, lungs and kidneys from oxidative stress under intermittent hypobaric hypoxia in rats. Biol Res. 2012; 45(1):81-85. doi: 10.4067/S0716-97602012000100011.

Acuña-Castroviejo D, Carretero М, Doerrier С, et al. Melatonin protects lung mitochondria from aging. Age (Dordr). 2012; 34(3):681-692. doi: 10.1007/s11357-011-9267-8.

Rozova KV. Vplyv normo- ta gipobarychnoi' gipoksii' na ul'trastrukturu tkanyny legen' i miokarda [Influence of normo- and hypobaric hypoxia on the ultrastructure of lung tissue and myocardium]. Fiziol. zhurn. 2008; 54(2):63-68. (in Ukrainian).

Okujama K, Dzhiang D, Ajhara K. Ul'trastrukturnye izmenenija v legkih na fone gipoksicheskoj terapii: ocenka terapevticheskoj jeffektivnosti kak raznovidnosti impul'snoj terapii [Ultrastructural changes in the lungs on the background of hypoxic therapy: evaluation of therapeutic efficacy as a form of pulse therapy]. Pul'monologija, 2004; 4:67-71. (in Russian).


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM




Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

https://pbn.nauka.gov.pl/sedno-webapp/search?search&searchCategory=WORK&filter.inJournal=36616

POL-index 2011 - 2014

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-32a319c5-a850-4f44-ba70-1418a6087655

BASE 2011 - 2014

https://www.base-search.net/Search/Results?lookfor=Journal+of+Health+Sciences+%28Radom%29&type=all&oaboost=1&ling=1&name=&thes=&refid=dcrespl&newsearch=1

http://elibrary.ru/contents.asp?titleid=37467

http://journal.rsw.edu.pl/index.php/JHS/issue/archive

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska, EuroPub database, NLM Catalog Result - NCBI, BASE, Russian Sciences Index, Arianta.

https://zdb-katalog.de/title.xhtml?idn=1080187162

https://europub.co.uk/journals/22625

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

https://www.ncbi.nlm.nih.gov/nlmcatalog/101679844

Find a library that holds this journal: http://worldcat.org/issn/23918306

 

PBN Poland

https://pbn.nauka.gov.pl/sedno-webapp/journals/49068

POL-index

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-c39c8169-88d2-45db-9e7f-d948ce9981c4

BASE

https://www.base-search.net/Search/Results?join=AND&bool0[]=AND&lookfor0[]=2391-8306&type0[]=all&page=11&l=pl&oaboost=1&refid=dcpagepl

Redaction, Publisher and Editorial Office

Publisher and Editorial Office
Department of Physical Culture,
Faculty of Earth Sciences and Spatial Management,
Nicolaus Copernicus University in Toruń, Poland
Address: Str. Lwowska 1, 87-100 Toruń, Poland

  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at https://dbh.nsd.uib.no/publiseringskanaler/erihplus/periodical/info?id=485984

Indexed in Index Copernicus Journals Master List.

http://journals.indexcopernicus.com/Journal+of+Education+Health+and+Sport,p24782242,3.html

ICV 2019 = 100.00 ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

RG Journal Impact: 0.18 *

*This value is calculated using ResearchGate data and is based on average citation counts from work published in this journal. The data used in the calculation may not be exhaustive.

RG Journal impact history

2020Available summer 2021
2018 / 20190.18
20170.07
20160.03

RG Journal impact over time

RG Journal impact
0.180.160.140.120.100.080.060.040.02201620172018
  

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (https://pbn.nauka.gov.pl/journals/36616)

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ) http://elibrary.ru/contents.asp?titleid=37467

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski

(http://www1.bg.us.edu.pl/bazy/czasopisma/czasop_full.asp?id=3595)

Partnerzy platformy czasopism