Rat peripheral blood leucocyte reaction in the age aspect on the background of metabolic syndrome experimental modeling

N. A. Shutova

DOI: http://dx.doi.org/10.12775/JEHS.2020.10.09.115


The purpose of the research is to investigate links between body mass index and changes in the leucocyte formula of peripheral blood in rats of different ages in experimental modeling of nongenetic alimentary obesity with subsequent MS development; to identify possible associations between these indicators in animals depending on age.

Contingent and research methods. Metabolic syndrome was reproduced according to the developed method in rats of different age groups by combining high-fat and high-carbohydrate diet in parallel with pharmacological correction of physiological satiation inhibition.

Results. It is determined that the mechanisms of low gradient chronic inflammation development in adipose tissues on the background of metabolic syndrome differ from classical inflammatory response mechanisms. Weight gain is associated with the activation of various leucocyte forms depending on age and degree of mass increase. Young rats have body mass index associated with the amount of peripheral blood neutrophils and more delayed in time; adult animals have body mass index associated with eosinophils, starting from the first month of metabolic syndrome development; old animals have body mass index associated with monocytes and lymphocytes, during the whole period of the trial. The dependence of inflammatory reaction symptoms on the age and sex of the animal on the background of obesity is confirmed by the present associative links (peripheral blood cell composition – animal’s age - body mass index) and can be determined later, as a marker factor for metabolic syndrome development.


leucocyte reaction; chronic low-grade inflammation; metabolic syndrome

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Mahbuba, S., Mohsin, F., Rahat, F., Nahar, J., Begum, T., & Nahar, N. (2018). Descriptive epidemiology of metabolic syndrome among obese adolescent population. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 12(3), 369-374. doi: 10.1016/j.dsx.2017.12.026

Srikanthan, K., Feyh, A., Visweshwar, H., Shapiro, J., & Sodhi, K. Systematic Review of Metabolic Syndrome Biomarkers: A Panel for Early Detection, Management, and Risk Stratification in the West Virginian Population. International Journal Of Medical Sciences; 2016. 13(1): 25-38. doi: 10.7150/ijms.13800

Divella, R., De Luca, R., Abbate, I., Naglieri, E., & Daniele, A. Obesity and cancer: the role of adipose tissue and adipo-cytokines-induced chronic inflammation. Journal Of Cancer; 2016. 7(15): 2346-2359. doi: 10.7150/jca.16884

Singer, K., Lumeng, C. The initiation of metabolic inflammation in childhood obesity. Journal Of Clinical Investigation; 2017. 127(1): 65-73. doi: 10.1172/jci88882

Belenkov, Y., Privalova, E., Kaplunova, V., Zektser, V., Vinogradova, N., & Ilgisonis, I. et al. Metabolic Syndrome: Development of the Issue, Main Diagnostic Criteria. Rational Pharmacotherapy In Cardiology; 2018. 14(5): 757-764. doi: 10.20996/1819-6446-2018-14-5-757-764

Obesity and overweight. Retrieved 3 March 2020, from http://www.who.int/en/news-room/fact-sheets/detail/obesity-and-overweight

Zhao, X., Gang, X., Liu, Y., Sun, C., Han, Q., & Wang, G. Using Metabolomic Profiles as Biomarkers for Insulin Resistance in Childhood Obesity: A Systematic Review. Journal Of Diabetes Research; 2016: 1-12. doi: 10.1155/2016/8160545

McLaughlin, T., Ackerman, S., Shen, L., & Engleman, E. Role of innate and adaptive immunity in obesity-associated metabolic disease. Journal Of Clinical Investigation; 2017. 127(1): 5-13. doi: 10.1172/jci88876

Rangel-Huerta, O., Pastor-Villaescusa, B., & Gil, A. Are we close to defining a metabolomic signature of human obesity? A systematic review of metabolomics studies. Metabolomics; 2019. 15(6): 2-10. doi: 10.1007/s11306-019-1553-y

Povoroznyuk, V., Gopkalova, I., & Grygorieva, N. Peculiarities of changes in the mineral density of the osseous tissue of albino Wistar rats depending on age and gender. The Problems Of Aging And Longevity (Kiev); 2011. 20(4): 393–401.

Kuzmina I., Shutova N., Nikolaieva O Sposib modeluvanna metabolichnogo sundromu v exsperimente. Patent Ukrainu №UE118945C2. 2019 March. 25. Kuzmina, І.Yu, Shutova, N.A, Nikolaieva, O.V. (2019) UA Patent No. 118945. Ukrainskyi instytut intelektualnoi vlasnosti (Ukrpatent). Retrieved from https://base.uipv.org/searchINV/search.php?action=viewdetails&IdClaim=256724 [in Ukrain].

Menshikov, V. Laboratornue metody issledovaniya v klinike. Spravochnic. Moskva: Мedicina; 1987: 368. [in Russian].

McPhee, J., & Schertzer, J. Immunometabolism of obesity and diabetes: microbiota link compartmentalized immunity in the gut to metabolic tissue inflammation. Clinical Science; 2015. 129(12): 1083-1096. doi: 10.1042/cs20150431111

Saltiel, A., & Olefsky, J. Inflammatory mechanisms linking obesity and metabolic disease. Journal Of Clinical Investigation; 2017. 127(1): 1-4. doi: 10.1172/jci92035

Gerasimenko, N. Lipidy, vospalenie i patologiya cheloveka: rol receptorov, aktiviruemyh proliferatorami peroksidom. Visnyk Problem Biolohii i Medytsyny; 2015. 1(118): 10-15. [in Russian].

Rudziak, P., Ellis, C., & Kowalewska, P. Role and Molecular Mechanisms of Pericytes in Regulation of Leukocyte Diapedesis in Inflamed Tissues. Mediators Of Inflammation; 2019: 1-9. doi: 10.1155/2019/4123605

Oliveira, S., Costa, C., Ferreira, S., & Cunha, F. Sephadex induces eosinophil migration to the rat and mouse peritoneal cavity: involvement of mast cells, LTB4, TNF-α, IL-8 and PAF. Inflammation Research; 2002. 51(3): 144-153. doi: 10.1007/pl00000286

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Journal of Education, Health and Sport formerly Journal of Health Sciences

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1223 Journal of Education, Health and Sport eISSN 2391-8306 7

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