Development of gingivitis in rats receiving oral applications of peroxide sunflower oil

A. V. Markov

DOI: http://dx.doi.org/10.12775/JEHS.2020.10.06.035

Abstract


Aim. Determine the condition of the gums in rats receiving oral applications of peroxide sunflower oil (PSO).

Methods. PSO was obtained by heating sunflower oil in the presence of Н2О2 at +180°С for 60 minutes. PSO in a dose of 0.5 ml per rat was applied to the oral mucosa for 3 or 5 days. The activity of elastase, urease, lysozyme, catalase and the content of MDA (malondialdehyde) were determined in the gum. The antioxidant-prooxidant API index was calculated by the ratio of catalase and MDA, and the degree of dysbiosis was calculated by the ratio of the relative activities of urease and lysozyme..

Results. After 5 days of PSO applications, an increase in elastase activity, urease, MDA content and the degree of dysbiosis against the background of a decrease in lysozyme activity, catalase and the API index was established.

Conclusion. Oral applications of PSO cause a decrease in the level of non-specific immunity, antioxidant defense, an increase in microbial seeding, the degree of dysbiosis and the development of gingivitis.


Keywords


periodontium; fat peroxidation; gingivitis; dysbiosis; lysozyme; antioxidant protection

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References


Levitsky AP, Potapova IL. Fatty food, fatty acids, Healthy sun-flower olive. Intern. Journ. Food a Nutrition sciences. 2015; 4(3): 15-20.

Levitsky AP. The pathophysiology of high-fat diet and ways to prevent its complications. Bulletin of XVII readings named after V. V. Podvysotsky, May 24-25, Odessa, 2018: 120-124. (in Russian)

Penumetcha M, Khan-Merihant N, Parthasarathy S. Enhanced solubilization and intestind absorption of cholesterol by oxidized linoleic acid. J. Lipid Res. 2002; 43(6): 895-903.

Labush IuZ, Markov AV, Zubachik VM [and others]. Mucosoprotective effect of lysozyme-forte in rats that received super-oxidized sunflower. Bulletin of Marine Medicine. 2018; 2(79): 75-79. (in Ukrainian)

Markov AV. Effect of over-oxidized sunflower oil on the condition of periodontium of rats. Bulletin of Dentistry. 2018; 28(2(103): 14-17. (in Ukrainian)

Bocharov A. V. The influence of flavancontent means on gut mucosa state at rats received peroxide sunflower oil. Journal of Education, Health and Sport. 2018; 8(8): 1200-1205.

Vasyuk V. L. Hepatoprotective action of flavancontent means at hepatopathy, caused by the peroxide sunflower oil. Bulletin of Marine Medicine. 2018; 1: 101-104. (in Russian)

Levitsky AP, Demyanenko SA. Hepato-oral syndrome. Simferopol, Tarpan, 2012: 140. (in Russian)

Levitsky AP, Denga OV, Makarenko OA [and others]. Biochemical markers of inflammation of oral cavity tissue: method guidelines. Оdеssа, KP OGT, 2010: 16. (in Russian)

Levitsky AP, Stefanov AV. The methods of the determination of the activity of elastase and its inhibitors: method guidelines. Kiev, GFK, 2002:15. (in Russian)

Stalnaya ID., Garishvili TG. The method of revelation of malonic dialdehyde with thiobarbituric acid. Moskow, Medicine, 1977: 66-68. (in Russian)

Girin SV. The modification of the method of the determination of catalase activity in biological substrates. Laboratory diagnosis. 1999; 4:45-46. (in Russian)

Gavrikova LM, Segen IT. Urease activity of oral liquid in patients with acute odontogenic infection of maxillo-facial part. Stomatology. 1996; The extra issue :49-50. (in Russian)

Levitsky AP. Lysozyme instead of antibiotics. Оdеssа, KP OGT, 2005: 74. (in Russian)

Levitsky AP, Makarenko OA, Denga OV [and others]. The experimental methods of restoration and estimation of the degree of dysbiosis in oral tissues. Bulletin of Dentistry. 2010; 2: 22-23. (in Russian)

Levitsky AP, Makarenko OA, Khodakov IV. Methods to investigate fats and oils. Odessa, KP OGT, 2015: 32. (in Russian)

Truhacheva NV. Mathematical Statistics in biomedical research using application package Statistica. Moskow, GJeOTAR-Media, 2012: 379. (in Russian)

Eggum B. Methods to evaluate utilization of proteins by animal. Moscow: Kolos, 1977: 189. (in Russian)

Markov AV, Labush IuZ, Zubachik VМ [and others]. Therapeutic and preventive action flavan-containing antidisbiotic means on a mucose membrane of the oral cavity of the rats, received peroxide sunflower oil. Phytotherapy. Chasis. 2018; 2: 33-35. (in Ukrainian)

Levitsky AP, Makarenko OA, Selivanskaуa IA [and others]. The experimental prophylaxis of the peroxide periodontitis by antidysbiotic means. Journal of Education, Health and Sport. 2017; 7(2): 682-693.


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