Influence of a high-fat diet on inflammation and dysbiotic processes in the gums and blood serum of rats with lincomycin dysbiosis

A. V. Markov



Background. To determine the effect on the periodontium and the whole organism of excessive consumption of palm oil, against the background of lincomycin dysbiosis.

Methods.The experiments were carried out on rats, divided into 3 groups: 1st ‒ control, 2nd and 3rd with dysbiosis, which was reproduced using lincomycin administered with drinking water at a dose of 60 mg / kg for 5 days. Rats of the 3rd group additionally received palm oil at a dose of16 g / kg with food. Rats were fed for 21 days. The activity of elastase, urease, lysozyme, catalase, and the content of malondialdehyde (MDA) and hyaluronic acid were determined in the blood serum and in the gums of rats.

Results. In rats, the administration of lincomycin caused an increase in the serum and gum activity of elastase and MDA content, an increase in urease activity and the degree of dysbiosis, but a significant decrease in lysozyme activity.

Conclusion. The introduction of lincomycin causes the development of systemic inflammation and generalized dysbiosis, the development of gingivitis and dysbiosis in the gums, especially when combining the administration of lincomycin with the consumption of palm oil.


periodontal; blood serum; dysbiosis; inflammation; antioxidants; fat nutrition; antibiotic

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Levitsky AP. Lysozyme instead of antibiotics. Оdеssа, KP OGT, 2005: 74. (in Russian)

Polunina TYe, Mayev IV. Clinic, diagnosis and correction of acute drug hepatitis. Therapist. 2007; 1: 88-89. (in Russian)

Novik GI, Astapovich NI, Ryabaya NE. The production of hydrolases and antibiotic resistance of lactobacilli and bifidobacilli. Applied Biochemistry and Microbiology. 2007; 43 (2): 184-192. (in Russian)

Levitsky AP, Makarenko OA, Tomilina TV [and others]. The experimental methods of reproduction ob immunodeficite conditions: method guidelines. Оdеssа, KP OGT, 2016: 20. (in Russian)

Levitsky AP, Makarenko OA, Demyanenko SA. Methods of experimental dentistry (teaching aid). Simferopol, Tarpan, 2018: 78. (in Russian)

Van der Kleij D, Yazdanbakhsh M. Control of inflamatory diseases by pathogens: lipids and the immune system. Eur. J. Immunol. 2003; 33: 2953-2963.

Velichko VI, Tkachuk VV, Levitsky AP. Development of dysbiosis in tissues of rats fed with a high fat food. Journal of Health Sciences. 2014; 4(12): 84-92. (in Russian)

Khodakov IV, Levitsky AP, Tkachuk VV [and others]. Prodysbiotic action of food fats with high content of palmitic acid. Byulleten' ХIV chteniy im. V. V. Podvysotskogo. Odessa, 2015: 200-201. (in Russian)

Titov VN. Excess palmitic fatty acid in food is the main cause of lipidosis of insulin-dependent cells: skeletal myocytes, cardiomyocytes, pediportal hepatocytes, Kupffer macrophages and pancreatic β-cells. Clinical laboratory diagnosis. 2016; 61(2): 68-77. (in Russian)

Levitsky AP, Makarenko OA, Denga OV [and others]. The experimental methods of restoration and estimation of the degree of dysbiosis in oral tissues. Announcer of stomatology. 2010; 2: 22-23. (in Russian)

Levitsky AP, Denga OV, Makarenko OA. [and others]. Biochemical markers of inflammation of oral cavity tissue: method guidelines. Оdеssа, KP OGT, 2010: 16. (in Russian)

Levitsky AP, Stefanov AV. The methods of the determination of the activity of elastase and its inhibitors: method guidelines. Kiev, GFK, 2002:15. (in Russian)

Stalnaya ID, Garishvili TG. The method of revelation of malonic dialdehyde with thiobarbituric acid. Moskva, Meditsina, 1977: 66-68. (in Russian)

Gavrikova LM, Segen IT. Urease activity of oral liquid in patients with acute odontogenic infection of maxillo-facial part. Stomatology. 1996; The extra issue :49-50. (in Russian)

Levitsky AP, Makarenko OA, Selivanskaya IA [and others]. Enzymatic methods for determination of oral dysbiosis for screening pro- and prebiotics: method guidelines. Кiev, GFC, 2007: 22. (in Russian)

Girin SV. The modification of the method of the determination of catalase activity in biological substrates. Laboratory diagnostics. 1999; 4: 45-46. (in Russian)

Asatiani VS. The new methods in biochemical photometry. Moskva, Nauka, 1965: 543. (in Russian)

Truhacheva NV. Mathematical Statistics in biomedical research using application package Statistica. Moscow, GJeOTAR-Media, 2012: 379. (in Russian)

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1223 Journal of Education, Health and Sport eISSN 2391-8306 7

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