Significance of lipid peroxidation processes in renal excretory dysfunction in cases of acute blood loss complicated by limb ischemia-reperfusion and its correction

V. V. Shatskyi, A. A. Hudyma

DOI: http://dx.doi.org/10.12775/JEHS.2019.09.11.027

Abstract


Introduction. Activation of lipid peroxidation processes, which are exacerbated in cases of limb ischemia-reperfusion associated with temporary application of a tourniquetis a key mechanism of acute blood loss. However, its significance in the pathogenesis of renal dysfunction is understudied. The information on the effectiveness of carbacetam administration under these conditions, which has an antioxidant effect, is lacking.

Objective. The aim of the research was to investigate the significance of lipid peroxidation processes in renal excretory dysfunction in cases of acute blood loss complicated by limb ischemia-reperfusion, and to evaluate the effectiveness of carbacetam for correction of these disorders.

Materials and methods. The experiments were performed on 108 nonlinear male rats weighing 160-180 g. All animals were divided into five groups: a control group and four experimental groups (6 rats in each).The first experimental group comprised the animals anesthetized with thiopental sodium, in which a two-hour limb ischemia was simulated with further reperfusion. In the second experimental group the acute blood loss in the amount of 20-22% of a circulating blood volume was simulated to the anesthetized animals by femoral vein dissection. In the third experimental group these injuries were combined. In the fourth experimental group the animals with acute blood loss complicated by limb ischemia-reperfusion were administered with carbacetam intraperitoneally at a dose of 5 mg per kilogram of animal weight for correction. In the control group, the animals were anesthetized with equivalent dose of thiopental sodium; a tourniquet was applied for 2 hours without stopping the blood flow, and subsequently studied in 1 hour.

In 1 and 2 hours, as well as in 1, 7and 14 days, the renal function was assessed in the experimental animals by means of the water loading test. Urine was collected for 2 hours and diures is was determined. After urine collection, the rats were anesthetized with thiopental sodium andtakenout of the experiment by total bloodletting from the heart. Concentrations of sodium in the urine and serum, as well as protein in the urine were determined. According to these data, sodium and protein excretion wasassessed. Additionally, the content of reagents with regard to thiobarbituric acid –TBA-active products of lipid peroxidation, was determined in the renal medulla.

Results and discussion. In cases of limb ischemia-reperfusion, acute blood loss and their combination, the content of TBA-active products of lipid peroxidation in the renal medulla increased the most in 1 day. Up to the 14thday, the index decreased, but did not reach the level of the control group and was significantly higher in the group of animals with acute blood loss complicated by limb ischemia-reperfusion. In cases of the simulated injuries, increased sodium and protein excretion was evidenced compareto the control group.

The highest increase in these parameters corresponded to the maximum activation of lipid peroxidation in the renal medulla in relation to the terms, and according to the amplitude increased from the group with limb ischemia-reperfusion to the group with acute blood loss and then to the group with combination of these disorders. This pattern was manifested the most significantly in 7-14 days of the reperfusion period. In case of carbacetam administration, the content of TBA-active products of lipid peroxidation and sodium excretionin the renal medulla significantly decreased in 7-14 days, compare to the animals without correction.

Protein excretion decreased in 14 days of carbacetam administration. Taking into account the pronounced antioxidant properties of carbacetam, the attained result proved the significance of activation of lipid peroxidation processes in cases of kidney damage after limb ischemia-reperfusion, acute blood loss and their combination.

Conclusions. With underlying limb ischemia-reperfusion, acute blood loss, and their combination, the sodium and protein excretion increase compare to the control group. The highest increase of these parameterstakes placein 1 day of the reperfusion period thatcorresponds to the maximal accumulation of TBA-active products of lipid peroxidation in the renal medulla in relation to the terms.

The amplitude of changes in the studied parameters increases from the group of animals with limb ischemia-reperfusion to that with acute blood loss, and then to the group with their combination that is the most pronounced in 7-14 days of the reperfusion period.

The carbacetam administration (at a dose of 5 mg per kilogram of animal body weight) causes a significant nephroprotective effect and up to the 14th day leads to a significant decrease in the content of TBA-active products of lipid peroxidation, reduced sodium and protein excretion in the renal medulla.


Keywords


blood loss; limb ischemia-reperfusion; kidney; lipid peroxidation; sodium and protein excretion

Full Text:

PDF

References


Preclinical medications research: guidelines. Edited by O.V. Stefanov. Kyiv: Avicenna, 2001. 528 p.

Kozak D. V. Effect of carbacetam on the antioxidant-prooxidant balance of the heart, lungs and liver tissues in the dynamics of polytrauma. Spytalna hirurhiia. 2014. Vol. 1 (65). P. 40-42.

Kuzminskyi I.V. Bile excretory function of the liver in cases of ischemic-reperfusion syndrome of extremities, closed trauma of abdominal organs complicated by massive blood loss. Aktualni problemy transportnoi medytsyny. 2018. Vol. 4 (54). P. 148-158.

Rohovyi Yu. Ye. Pathophysiology of hepatorenal syndrome at the polyuric stage of sulemic nephropathy. Chernivtsi: Misto, 2012. 200 p.

Televiak A. T. Dynamics of lipid peroxidation and antioxidant protection parameters in the muscle tissue of ratshind limbs in cases of ischemic-reperfusion syndrome development (experimental study). Zdobutky klinichnoi I eksperymentalnoi medytsyny. 2018. Vol. 3 (35). P. 132-139.

Tsymbaliuk H.Yu. Daytime diuresis of the kidneys in cases of ischemic-reperfusion limb syndrome, injuries of abdominal organs complicated by hypovolemic shock and their combination in the early period of traumatic disease. Zdobutky klinichnoi I eksperymentalnoi medytsyny. 2018. Vol. 3 (35). P. 163-169.

Shatskyi V. V., Stakhiv O. V., Maksymiv R. V., Strelbytska I. V. Dynamics of the functional state of the kidneys in cases of limb ischemia-reperfusion, acute blood loss and their correction. Emergency care in accidents: National Research and Training Conference, February 20-24, 2019.Ternopil: Ukrmedknyha, 2019. P. 40-41.

Sheiman D. A. Pathophysiology of the kidney. St. Petersburg: Binom, 2017. 208 p.

An evidence-based prehospital guideline for external hemorrhage control: American College of Surgeons Committee on Trauma. / E. M. Bulger, D. Snyder, K. Schoelles et al. Prehospital Emergency Care. 2014. № 18. P.163—173.

Clasper J. C., Brown K. V., Hill P. Limb complications following pre-hospital tourniquet use., J R Army Med Corps. 2009. Vol. 155(3). P. 200-202.

Comparison of changes in markers of muscle damage induced by eccentric exercise and ischemia/reperfusion / Q.S. Su, J. G. Zhang, R. Dong [et al.]. Scand J Med Sci Sports. 2010. Vol. 20, N 5. Р. 748-756

Concentration decrease of nitric oxide in the postischemic muscle is not only caused by the generation of O2- / F. Stoffels, F. Lohöfener, М. Beisenhirtz [et al.] Microsurgery. 2007. Vol.27, N 6. Р. 565–568.

Efficacy of Prehospital Application of Tourniquets and Hemostatic Dressings To Control Traumatic External Hemorrhage. – [available at] https://www.ems.gov/pdf/research/Studies-and-Reports.pdf

Global differences in causes, management, and survival after severe trauma: the recombinant activated factor VII phase 3 trauma trial / M. C. Christensen, M. Parr, B. J. Tortellaet al. Journal of Trauma. 2010. Vol. 69 (2). P. 344-352.

Morsey H., Aslam M., Standfield N. Patients with critical ischemia of the lower limb are at risk of developing kidney dysfunction. Am J Surg. 2003. Vol.185. P. 360-363.


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM




Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

https://pbn.nauka.gov.pl/sedno-webapp/search?search&searchCategory=WORK&filter.inJournal=36616

POL-index 2011 - 2014

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-32a319c5-a850-4f44-ba70-1418a6087655

BASE 2011 - 2014

https://www.base-search.net/Search/Results?lookfor=Journal+of+Health+Sciences+%28Radom%29&type=all&oaboost=1&ling=1&name=&thes=&refid=dcrespl&newsearch=1

http://elibrary.ru/contents.asp?titleid=37467

http://journal.rsw.edu.pl/index.php/JHS/issue/archive

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska, EuroPub database, NLM Catalog Result - NCBI, BASE, Russian Sciences Index, Arianta.

https://zdb-katalog.de/title.xhtml?idn=1080187162

https://europub.co.uk/journals/22625

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

https://www.ncbi.nlm.nih.gov/nlmcatalog/101679844

Find a library that holds this journal: http://worldcat.org/issn/23918306

 

PBN Poland

https://pbn.nauka.gov.pl/sedno-webapp/journals/49068

POL-index

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-c39c8169-88d2-45db-9e7f-d948ce9981c4

BASE

https://www.base-search.net/Search/Results?join=AND&bool0[]=AND&lookfor0[]=2391-8306&type0[]=all&page=11&l=pl&oaboost=1&refid=dcpagepl

Redaction, Publisher and Editorial Office

Publisher and Editorial Office
Department of Physical Culture,
Faculty of Earth Sciences and Spatial Management,
Nicolaus Copernicus University in Toruń, Poland
Address: Str. Lwowska 1, 87-100 Toruń, Poland

  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at https://dbh.nsd.uib.no/publiseringskanaler/erihplus/periodical/info?id=485984

Indexed in Index Copernicus Journals Master List.

http://journals.indexcopernicus.com/Journal+of+Education+Health+and+Sport,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

RG Journal Impact: 0.18 *

*This value is calculated using ResearchGate data and is based on average citation counts from work published in this journal. The data used in the calculation may not be exhaustive.

RG Journal impact history

2020Available summer 2021
2018 / 20190.18
20170.07
20160.03

RG Journal impact over time

RG Journal impact
0.180.160.140.120.100.080.060.040.02201620172018
  

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (https://pbn.nauka.gov.pl/journals/36616)

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ) http://elibrary.ru/contents.asp?titleid=37467

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski

(http://www1.bg.us.edu.pl/bazy/czasopisma/czasop_full.asp?id=3595)

Partnerzy platformy czasopism