Lipopolysaccharide increases lysozyme adhesion to chitin

A. P. Levitsky, L. M. Mudrik, L. N. Khromagina, T. A. Sevostianova, I. A. Selivanskaia

DOI: http://dx.doi.org/10.12775/JEHS.2019.09.11.021

Abstract


Background. To study the effect of lipopolysaccharide on the adsorption of lysozyme on chitin.

Methods. Egg white lysozyme, lipopolysaccharide and chitin were used. The lyophilized cells of M. lyzodeikticus were used as a lysozyme substrate. Lysozyme activity was determined by the bacteriolytic method using different concentrations (8-512 μg/ml). Lysozyme adsorption on chitin was determined by the loss of lysozyme from solution after shaking with chitin. The effect of lipopolysaccharide (0.6-40 μg/ml) on lysozyme adsorption was evaluated by the loss of lysozyme from the solution after shaking with 50 mg of chitin.

Results. In determining the activity of lysozyme by the bacteriolytic method, the linear nature of the dependence of activity on the concentration of the enzyme is observed only at low concentrations (8-100 μg / ml). Lipopolysaccharide does not affect the bacteriolytic activity of lysozyme, however, dose-dependently increases the adsorption of lysozyme on chitin.

Conclusion. Lipopolysaccharide increases the adsorption of lysozyme on chitin.


Keywords


lysozyme, lipopolysaccharide; chitin; bacteriolysis; adsorption of lysozyme on chitin

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References


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