The influence of alliin on actin cytoskeleton reorganization in H1299 cell line in the presence of low doses of doxorubicin
DOI:
https://doi.org/10.12775/MBS.2015.004Keywords
H1299 cell line, alliin, doxorubicin, F-actin, cell death, apoptosis, mitotic catastropheAbstract
Alliin, garlic amino acid, has been thought to be pharmacologically inactive. However, several studies carried out on the cell cultures and the mammalian models demonstrated antioxidative and anticancer properties of alliin. Doxorubicin (DOX) is a well-known cytostatic agent, but often presented the side effects. Therefore, lowering a dose of DOX may reduce a negative influence of this drug on patients. The combined effect of DOX and alliin on the induction of cell death and the reorganization of actin cytoskeleton have not been yet tested in the human non-small cell lung cancer H1299 cells, thus the aim of the study was to evaluate the effects of DOX and alliin on the induction of cell death, morphological and ultrastructural alterations, as well as the reorganization of F-actin in the H1299 cells, using image cytometry and light, fluorescence and transmission electron microscopy. The experimental results presented here indicate that alliin enhances DOX impact on H1299 cells in a dose-dependent manner by increasing the percentages of apoptotic cells and reducing the cell viability. It also led to F-actin reorganization and both morphological and ultrastructural alternations. Alliin can support the tumoricidal effect of DOX but this potential therapeutic use should be examined in further studies.References
Chanda S, Kushwaha S, Tiwari RK: Garlic as food, spice and medicine: a perspective. J Pharm Res, 2011; 4: 1857-1860.
Stoll A, Seebeck E: Chemical investigations of alliin, the specific principle of garlic. Adv Enzymol Relat Area Mol Biol, 1951; 11: 377–400.
Mousa AS, Mousa SA: Anti-Angiogenesis Efficacy of the Garlic Ingredient Alliin and Antioxidants: Role of Nitric Oxide and p53. Nutr. Cancer, 2005; 53: 104–110.
Sangeetha T, Quine SD: Preventive effect of S-allyl cysteine sulphoxide (Alliin) on mitochondrial dysfunction in normal and isoproterenol induced cardiotoxicity in male Wistar rats: a histopathological study. Mol Cell Biochem,
; 328: 1-8.
Augusti KT, Sheela CG: Antiperoxide effect of S-allyl cysteine sulfoxide, an insulin secretagogue, in diabetic rats. Experientia, 1996; 52: 115-119.
Abbehausen C, Sucena SF, Lancellotti M et al.: Synthesis, spectroscopic characterization, DFT studies, and antibacterial and antitumor actvities of a novel water soluble Pd(III) complex with L-alliin. J Mol Struct, 2013; 1035: 421-426.
Nasim SA, Bhupinder D, Rashmi K et al.: Alliin obtained from leaf extract of garlic grown under in situ conditions possess higher therapeutic potency as analyzed in alloxan-induced diabetic rats. Pharm Biol, 2011; 49: 416-421.
Singhal SS, Wickramarachchi D, Singhal J et al.: Determinants of differential doxorubicin sensitivity between SCLC and NSCLC. FEBS Lett, 2006; 580: 2258–64.
Eom, YW, Kim MA, Park SS et al.: Two distinct modes of cell death induced by doxorubicin: apoptosis and cell death through mitotic catastrophe accompanied by senescence-like phenotype. Oncogene. 2005; 24: 4765-77.
Park SS, Eom YW, Choi KS: Cdc2 and Cdk2 play critical roles in low dose doxorubicin-induced cell death through mitotic catastrophe but not in high dose doxorubicin-induced apoptosis.Biochem Biophys Res Commun 334: 1014-1021, 2005.
Litwiniec A, Grzanka A, Helmin-Basa A et al.: Features of senescence and cell death induced by doxorubicin in A549 cells: organization and level of selected cytoskeletal proteins. J. Cancer Res. Clin. Oncol. 2010; 136: 717-36.
Tacar O, Sriamornsak P, Dass CR: Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems. J Pharm Pharmacol, 2013; 65: 157–170.
Dos Remedios CG, Chhabra D, Kekic, et al.: Actin Binding Proteins: Regulation of Cytoskeletal Microfilaments. Physiol Rev, 2003; 83: 433–73.
Pollard TD, Blanchoin L, Mullings RD: Molecular Mechanisms Controlling Actin Filament Dynamics in Nonmuscle Cells. Annu Rev Biophys Biomol Struct, 2000; 29: 545–76.
Grzanka D, Gagat M, Izdebska, M: Actin is required for cellular death. Acta Histochem, 2013; 115: 775–782.
Pollard TD, Cooper JA: Actin, a Central Player in Cell Shape and Movement. Science, 2009; 326: 1208-1212.
Chhabra ES, Higgs HN: The many faces of actin: Matching assembly factors with cellular structures. Nature Cell Biology, 2007: 9; 1110–1121.
Cisterna B, Necchi D, Prosperi E, et al.: Small ribosomal subunits associate with nuclear myosin and actin in transit to the nuclear pores. FASEB J. 2006;20:1901-1903.
Wu XJ, Kassie F, Mersch-Sundermann V: The role of reactive oxygen species (ROS) production on diallyl disulfide (DADS) induced apoptosis and cell cycle arrest in human A549 lung carcinoma cells. Mutat Res, 2005; 579:115–24.
Chung LY: The antioxidant properties of garlic compounds: allyl cysteine, alliin, allicin and allyl disulfide. J Med Food, 2006; 9: 205-213.
Louis XL, Murphy R, Thandapilly SJ, et al.: Garlic extracts prevent oxidative stress, hypertrophy and apoptosis in cardiomyocytes: a role for nitric oxide and hydrogen sulfide. BMC Complement Altern Med, 2012; 12:140.
Grzanka D, Domaniewski J, Grzanka A: Effect of doxorubicin on actin reorganization in Chinese hamster ovary cells. Neoplasma, 2005; 52: 46-51.
Chrustek A, Izdebska M, Hałas M, et al.: The influence of doxorubicin on nuclear and cytoplasmic pool of F-actin in the A549 cell line. Med.Biol.Sci, 2014; 28: 11-17.
Rathos MJ, Khanwalkar H, Joshi K et al.: Potentiation of in vitro and in vivo antitumor efficacy of doxorubicin by cyclin-dependent kinase inhibitor P276-00 in human non-small cell lung cancer cells. BMC Cancer. 2013; 13: 29.
Grzanka A, Grzanka D, Orlikowska M. Cytoskeletal reorganization during process of apoptosis induced by cytostatic drugs in K-562 and HL-60 leukemia cell lines. Biochem Pharmacol. 2003;66:1611-1617.
Bunnell TM, Burbach BJ, Shimizu Y, et al. β-Actin specifically controls cell growth, migration, and the G-actin pool. Mol Biol Cell, 2011; 22: 4047-4058.
Wang, Y, George SP, Srinivasan K et al.: Actin reorganization as the molecular basis for the regulation of apoptosis in gastrointestinal epithelial cells. Cell Death Differ, 2012; 19: 1514–24.
Grzanka D, Marszałek A, Gagat M et al.: Doxorubicin-induced F-actin reorganization in cofilin-1 (nonmuscle) down-regulated CHO AA8 cells. Folia Histochem Cytochem, 2010; 48: 377-86.
Pawlik A, Nowak JM, Grzanka D et al.: Hyperthermia induces cytoskeletal alterations and mitotic catastrophe in p53-deficient H1299 lung cancer cells. Acta Histochem, 2013; 115: 8-15.
Downloads
Published
How to Cite
Issue
Section
Stats
Number of views and downloads: 315
Number of citations: 0
