THE EFFECTS OF CAFFEINE ON CYTOSKELETON IN CHO AA8 CELL LINE
DOI:
https://doi.org/10.12775/MBS.2014.012Keywords
caffeine, vimentin, F-actin, tubulin, apoptosis, mitotic catastropheAbstract
The caffeine is one of the most widely consumed alkaloids, which is found in energy drinks, coffee, tea, cocoa and chocolate. This neuroactive substance is a component of the daily diet of millions people in the world. For many years, the attention of researches focused on the effects of caffeine on the living organism, especially in the context of physiological processes. Today, the mechanism of the action of caffeine is the object of the studies. The cytoskeletal proteins play an important role in cell death. In our work, we analyzed changes in vimentin, F-actin and β-tubulin cytoskeleton. Furthermore, alternations in morphology and ultrastructure were also evaluated. We used fluorescence and light microscopy and transmission electron microscopy as well. Our data showed that the treatment with caffeine resulted in decreased in the survival of CHO cells. Moreover, we noticed two types of cell morphology: giant, multinucleated cells and shrunken cells with chromatin condensation. This investigation suggested that caffeine may induce apoptosis and mitotic catastrophe cell death.
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