@article{Popadynets’_Bilas_Flyunt_Badiuk_Zukow_Gozhenko_Popovych_2020, title={Features of the condition of the neuroendocrine-immune complex in different constellations of entropies of morpho-functional immune subsystems in rats}, volume={10}, url={https://apcz.umk.pl/JEHS/article/view/JEHS.2020.10.08.025}, DOI={10.12775/JEHS.2020.10.08.025}, abstractNote={<p class="MsoNormal" style="text-align: justify; text-justify: inter-ideograph;" align="justify"><strong><span style="font-family: ’Times New Roman’;">Background.</span></strong><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> We have previously shown that the Entropy </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">(E) </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">of the </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">Thymocytogram (</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">T</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">), Splenocytogram </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">(S) as well as </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">Leukocytogram </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">(L) </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">and Immunocytogram </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">(I) </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">of the blood </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">in rats are virtually independent of each other</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">. The </span><strong><span style="font-family: ’Times New Roman’;">purpose</span></strong><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> of this study was to clarify the characteristics of the condition</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> of the neuroendocrine-immune complex in different constellations of E</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">(s)</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">of these four morpho-functional immune subsystems</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">. </span><strong><span style="font-family: ’Times New Roman’;">Materials and methods.</span></strong><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">Experiment was performed on </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">10</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">8 healthy Wistar rats </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">(48 male and 60 </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">female</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">) weighing </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">240-290 g divided into </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">8</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> groups. Animals of the first group remained intact</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">.</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> Instead, the other rats received various balneofactors for 6 days</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">.</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> The day after the completion of the drinking</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">/application</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> course </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">the</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">parameters</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">of</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">neuroendocrine</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">-</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">immune</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">complex</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">were</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">registered</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">. </span><strong><span style="font-family: ’Times New Roman’;">Results.</span></strong><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> The method of cluster analysis created four homogeneous groups of rats, significantly different from each other in the constellation of E of four morpho-functional immune subsystems</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">. </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">The m</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">embers of the first cluster (n=31) are characterized by elevated levels of E (Z±SE) of I (1</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">12±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">16), T (1</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">00±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">14) and L (0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">88±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">12) at the normal </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">E </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">level of S (0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">14±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">16). The members of the second cluster (n=21) are characterized by reduced E </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">level of </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">L (-1</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">59±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">16) in combination with increased levels of I (0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">80±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">17) and T (0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">81±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">15) at the normal </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">E </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">level of S (0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">13±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">16). A characteristic feature of the members of the third cluster (n=28) is reduced E</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> of </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">T (-1</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">01±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">17) at normal </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">E </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">levels of S (-0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">33±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">23), I (-0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">19± 0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">14 ) and L (0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">35±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">20). The last cluster (n=28) is characterized by reduced E </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">of </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">I (-1</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">36± 0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">14), while the E of other immune subsystems are at the borderline levels: L (-0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">52±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">16), S (0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">49±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">15), T (0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">60±0</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">,</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">15).</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">The method of discriminant analysis revealed 15 parameters of the neuroendocrine-immune complex, the set of which four clusters of Entropies differ from each other with an accuracy of 95%. </span><strong><span style="font-family: ’Times New Roman’;">Conclusion</span></strong><strong><span style="font-family: ’Times New Roman’;">.</span></strong><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> Each constellation of independent Entropies of the </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">thymocytogram, splenocytogram, immunocytogram and leukocytogram</span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;"> </span><span style="mso-spacerun: ’yes’; font-family: ’Times New Roman’; font-size: 12,0000pt;">is accompanied by a specific constellation of parameters of the neuroendocrine-immune complex which indicates their natural functional relationships.</span></p>}, number={8}, journal={Journal of Education, Health and Sport}, author={Popadynets’, Oleksandr and Bilas, Volodymyra and Flyunt, Igor-Severyn and Badiuk, Nataliya and Zukow, Walery and Gozhenko, Anatoliy and Popovych, Igor}, year={2020}, month={Aug.}, pages={214–229} }