Mechanism of stress heart damage in the animals of different sensitivity to hypoxia
Keywordscytokines, stress, myocardium, hypoxia sensitivity, sex, rats
Hypoxia and resistance to it is the main in today data sitiuayion in the world.
The aim of the study was to determine level of myocardium damage and corresponding of it with cytokines level in high- and low-resistance to hypoxic hypoxia rats (HR, LR) in different models of immobilization stress.
Material and methods of investigation. The experiments were performed on 144 outbred HR and LR aged 5.5-6 months, dividing into 3 groups – control and 2 with different model of immobilizing stress. It was determined morphological changes of myocardium and concentration of interleukins 1beta, 2, 4, 6, 10 (IL-1beta, IL-2, IL-4, IL-6, IL-10), tumor necrosis factor alpha (TNF-alpha) in the blood serum.
Results. The morphological investigation showed the bigger damage effect in LR animal, than in HR rats. Damage effects were higher in males, than in females. The stress repeating every 72 hours (stress 2) had higher damage in myocardium, than stress repeating every 24 hours (stress 1). We corresponds the results with cytokines level. High congenital resistance to hypoxia is associated with an increased content of anti-inflammatory cytokines (in males IL-4, in females IL-10). Intact males, compared with females, have a higher production of pro-inflammatory cytokines (IL-6, TNF-alpha).
At stress1 inHR males, compared with HR females, were lower contents of IL-10, higher level of IL-1beta, IL-4, IL-6, TNF-alpha. At stress1 inLR males, compared with LR females, were lower contends of IL-10, IL-2. The morphological changes were more in males, that’s why higher level of anti-inflammatory cytokines IL-10 really has protection in HR and LR females’ animals. At stress2 inHR male, compared with HR female, were lower contents of IL-1beta and IL-10, higher IL-2 and IL-6. At stress2 inLR males, compared with LR females, were higher level of IL-10, IL-6, lower contents of IL-2, IL-4. The morphological changes were more in males, that’s why pro-inflammatory cytokines IL-6 really take place in damage of myocardium in males with different resistance animals.
Conclusion. High congenital resistance to hypoxia is associated with smaller destroying of the myocardium layer in immobilizing stress, which correspond with increased content of anti-inflammatory cytokines IL-4 and, IL-10. Higher damage of myocardium in immobilizing stress correspond with higher production of pro-inflammatory cytokines (IL-6, TNF-alpha).
Network between Cytokines, Cortisol and Occupational Stress in Gas and Oilfield Workers / M. Reale, E. Costantini, C. D’Angelo, L. Coppeta, R. Mangifesta, S. Jagarlapoodi, M. Di Nicola and L. Di Giampaolo // International Journal of Molecular Sciences. 2020;21:1118 doi:10.3390/ijms21031118
The impact of acute stress on hormones and cytokines and how their recovery is affected by music-evoked positive mood / S. Koelsch, A. Boehlig, M. Hohenadel, I. Nitsche, K. Bauer & U. Sack // Sci. Rep. 2016;6: 23008.
Rohleder N., Aringer M., Boentert M. Role of interleukin-6 in Stress, Sleep, and Fatigue // Ann N Y Acad Sci. 2012;1261:88–96.
Niraula A., Witcher K. G., Sheridan J. F., Godbout J. P. Interleukin-6 Induced by Social Stress Promotes a Unique Transcriptional Signature in the Monocytes That Facilitate Anxiety // Biol Psychiatry. 2019;15;85(8):679–689.
Yang R., Lin Q., Gao H.B., Zhang P. Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells // Braz J Med Biol Res. 2014;47(2 ):101–109.
Effects of Immobilization Stress and Adrenomodullin on Interleukin-10 Levels in Some Rat Tissues / Gadalla A., Al Gendy A., Hashim A. M., Amer M. M., Sabry O. M. O. // Bull. Egypt. Soc. Physiol. Sci. 2013;33 (2):161–171.
Altitude Omics: The Integrative Physiology of Human Acclimatization to Hypobaric Hypoxia and Its Retention upon Reascent / A. W. Subudhi, N. Bourdillon, J. Bucher [et al.] // PLoS One. 2014;9(3):e92191.
Gibson O. R., Taylor L., Watt P. W., Maxwell N. S. Cross-Adaptation: Heat and Cold Adaptation to Improve Physiological and Cellular Responses to Hypoxia // Sports Med. 2017;47(9):1751–1768.
Glutamate receptors in the nucleus tractus solitarius contribute to ventilatory acclimatization to hypoxia in rat / M. E. Pamenter, J. A. Carr, A. Go [et al.] // J. Physiol. 2014;592(Pt 8):1839–1856.
Colleen G. Julian, Lorna G. Moore Human Genetic Adaptation to High Altitude: Evidence from the Andes // Genes (Basel). 2019;10(2):150.
Shimoda L. A. Let's Talk about Sex: A Novel Mechanism by Which Estrogen Receptor β Limits Hypoxia-Inducible Factor Expression in Pulmonary Endothelial Cells // Am. J. Respir. Cell Mol. Biol. 2018;59(1):11-12.
Miller A. J., Cui J., Luck J. C., Sinoway L. I., Muller M. D. Age and sex differences in sympathetic and hemodynamic responses to hypoxia and cold pressor test // Physiol. Rep. 2019;7(2):e13988.
Souza G. M. P. R., Amorim M. R., Moraes D. J. A., Machado B.H. Sex differences in the respiratory-sympathetic coupling in rats exposed to chronic intermittent hypoxia // Respir. Physiol. Neurobiol. 2018;256:109-118.
Coronavirus and Cardiovascular Disease, Myocardial Injury, and Arrhythmia / G. Giustino, S. P. Pinney, A. Lala, V. Y. Reddy, H. A. Johnston-Cox, J. I. Mechanick, J. L. Halperin and V. Fuster // J Am Coll Cardiol. 2020;76(17):2011-2023.
Berezovskij V. A. Hypoxia and individual peculiarities of reactivity. – K.: Scientific thought, 1978;2-216. [in Russian]
Kulynskyi V. Y., Olkhovskyi Y. A. Two adaptation strategies in adverse conditions: resistant and tolerant. The role of hormones and receptors. Advances of modern biology. 1992;112;697–711 [in Russian].
Sennikov S. V., Silkov A, N. Methods for determination of cytokines // Cytokines and inflammation. 2005;4(1):22–27. [in Russian].
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