THE INFLUENCE OF DOXORUBICIN ON NUCLEAR AND CYTOPLASMIC POOL OF F-ACTIN IN THE A549 CELL LINE
DOI:
https://doi.org/10.12775/MBS.2014.010Keywords
A549 cell line, doxorubicin, cell death, apoptosis, mitotic catastrophe, F-actinAbstract
The cytoskeleton as an intracellular system plays an important role in the proper functioning of the cell. F-actin is one of the components, which built this structure. Microfilaments are involved in cell shape maintenance, polarity and cell motility. The aim of the present study was to determine the effect of doxorubicin on the actin reorganization and type of induced cell death in A549 cell line. In order to examine F-actin, the material was evaluated by the confocal and classical fluorescence microscope. Furthermore, changes in morphology and ultrastructure were analyzed by a light and transmission electron microscopy. The obtained data showed that doxorubicin causes a dosedependent decrease in A549 cell viability. Moreover, the treatment with doxorubicin resulted in the reorganization of F-actin as well as the induction of apoptosis and mitotic catastrophe in the non-small lung cancer cells. In addition, the existence of actin in the nucleus was confirmed.
References
Alberts B, Bray D, Hopkin K, Johnson A, Lewin J, Ruff M, Roberts K, Walter P: Podstawy biologii komórki. Wydawnictwo Naukowe PWN, Warszawa, 2005; 573-606.
Chen Q, Wu LJ, Wu YQ, Hualu G, Jiang ZY, Zhan WJ, Jie Y, Zhou JY: Molecular mechanism of trifluoperazine induces apoptosis in human A549 lung adenocarcinoma cell lines. Mol Med Rep, 2009; 2: 811-817.
Clarke PGH: Developmental cell death morphological diversity and multiple mechanisms. Anat Embryol, 1990; 181: 195-213. DOI:
Dos Remedios CG, Chhabra D, Kekie M, Dwdova IV, Tsubakihara M, Berry DA, Nosworthy NJ: Actin binding proteins: regulation of cytoskeletal microfilaments. Physiol Rev, 2003; 83: 433-473.
Draber P, Sulimerko V, Draberova E: Cytoskeleton in mast cell signaling. Front Immunol, 2012; 3(130): 1-18.
Earnshaw WC, Polland TD: Cell biology. Saunders Elsevier, 2008; 603-850.
Elmore S: Apoptosis: a review of programmed cell death.
Toxical Pathol, 2007; 35(4): 455-516.
Fadeel B, Orenius S: Apoptosis a basic biological phenomenon with wide-ranging implications in human disease. J Intern Med, 2005; 258: 479-517.
Fletcher DA, Mullins RD: Cell mechanics and the cytoskeleton. Nature, 2010; 463(7280): 485-492.
Gettemans J, Impe KV, Delanote V, Hubert T, Vandekerokhove J, Corte VD: Nuclear Actin binding proteins as modulators of gene transcription. Traffic, 2005; 6: 847-57.
Grzanka A, Cichos J, Żuryń A: Ocena obecności aktyny na terenie jadra komórkowego komórek białaczki ludzkiej HL-60 hodowanych w obecności etopozydu i doksorubicyny. Annales Academicae Bydgostiensis, 2004; 18(4): 59-65.
Grzanka A, Grzanka D, Orlikowska M: Fluorescence and ultrastuctural localization of actin distribution patterns in the nucleus of HL-60 and K-562 cell lines treated with cytostatic drugs. Oncol Rep, 200; 11: 765-770.
Grzanka D, Domaniewski J, Grzanka A: Effect of doxorubicin on actin reorganization in Chinese hamster over cells. Neoplasma, 2005; 52(1): 46-51.
Grzanka D, Grzanka A, Izdebska M, Gackowski L, Stępień A, Marszałek A: Actin reorganization in CHOAA8 cells undergoing mitotic catastrophe and apoptosis induced by doxorubicin. Oncol Rep, 2010; 23: 655-663.
Grzanka D, Marszałek A, Izdebska M, Gackowski L, Szczepański MA: Actin cytoskeleton reorganization correlate with cofilin nuclear expression and ultrastructural changes in CHOAA8 cell line after apoptosis and mitotic catastrophe induction by doxorubicin. Ultrastructural Pathol, 2011; 35(3): 130-138.
Haake AR, Polakowski RR: Cell death by apoptosis in epidermal Biology. J Invest Dermatol, 1993; 10(2): 107-112. DOI:
Jeyaseelan R, Poizat C, Wo HY, Kedes L: Molecular mechanisms of doxorubicin- induced cardiomyopathy. J Biol Chem, 1997; 272(9): 5828-5832.
Kerr JFR, Wyllie AH, Currie AR: Apoptosis a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer, 1972; 26: 239-57.
Litwiniec A, Grzanka A, Helmin-Basa A, Gackowska L, Grzanka D: Features of senescence and cell death induced by doxorubicin in A549 cells organization and level of selected cytoskeletal proteins. Cancer Res Clin Oncol, 2010; 136: 717-736.
Lodish H, Berk A, Zipursky L, Matsudain P, Baltimore D, Darnell J: Mol Cell Biol. New York, 2000.
Migocka-Patrzałek M, Malicka-Błaszkiewicz M: Aktyna w jądrze komórkowym. Post Biol, 2009; 55(2): 232-238.
Nowak JM, Grzanka A, Gagat M, Żuryń A: The influence of cotinine on the non-small-cell lung cancer line A549: Post Hig Med Dosw, 2009; 63: 1-7.
Pederson T, Aebi U: Nuclear actin extends with no contraction in sight. Mol Biol Cell, 2005; 16: 5055-60. DOI:
Podleski JK, Chwalibogowsa-Podlewskaa A: Leki współczesnej terapii. Medical Tribune Polska, 2009; 270-271.
Ramage L, Jones AC, Whelan CJ: Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro. J Inflamm, 2006; 3(3): 1-11.
Singal PK, Liskovic N: Doxorubicin- induced cardiomyopathy. N Engl J Med, 1998; 339(3): 900-905.
Stępień A, Izdebska M, Grzanka A: Rodzaje śmierci komórki. Post Hig Med. Dośw, 2007 61; 420-428.
Desouza M., Gunning P.W., Stehn J.R.: The actin cytoskeleton as a sensor and mediator of apoptosis. BioArchitecture, 2012; 2: 75-87.
Olave IA, Reck-Peterson SL, Crabtree GR: Nuclear actin and actin-related proteins in chromatin remodeling. Annu Rev Biochem 2002 71:755–781.
Downloads
Published
How to Cite
Issue
Section
Stats
Number of views and downloads: 376
Number of citations: 0