Ultraviolet radiation (UV) induces f-action rearrangement and cell death in the A549 human lung cancer cell line
DOI:
https://doi.org/10.12775/MBS.2014.003Keywords
sport of people with disabilities, a medical college, a physical education college, male students, female students, profession, viewsAbstract
The human lung cancer cell line, A549, was employed to investigate the effect of UV radiation on the actin cytoskeleton organization in relation to its potential involvement in cell death processes. The light and electron microscopy analysis was performed to find the morphological signs of cell death in UV-treated A549 cells. In turn, the F-actin architecture was determined using fluorescence microscopy after phalloidin-Alexa Fluor 488 staining. We observed apoptosis as well as mitotic catastrophe-like morphological changes in A549 cells exposed to UV radiation. We also found the vacuoles in the cytoplasm of UV-exposed A549 cells, which are considered to be indicative of autophagy. All morphological effects of UV radiation were time-dependent. Furthermore, alterations in cell morphology corresponded with actin cytoskeleton reorganization. F-actin was presented in the form of dense ring-like structures surrounding the nuclei of cells with apoptotic-like phenotype. Moreover, in some of these cells depolymerization of F-actin occurred. On the other hand, the enlarged cells exhibited strongly expanded actin network. Our study revealed that ultraviolet radiation induced F-actin reorganization, which was accompanied by the characteristic apoptotic features. These results also suggest that UV induces not only the apoptosis but also the non-apoptotic cell deaths and in each of these processes reorganization of actin cytoskeleton is essential.
References
Armstrong B.K., Kricker A.: The epidemiology of UV induced skin cancer. J. Photochem. Photobiol., 2001; 63: 8-18
Juzeniene A., Moan J.: Beneficial effects of UV radiation other than via vitamin D production. Dermato- Endocrinology, 2012; 4: 109–117
Opländer C., Volkmar C.M., Paunel-Görgülü A., et al.: Whole body UVA irradiation lowers systemic blood pressure by release of nitric oxide from intracutaneous photolabile nitric oxide derivates. Circ. Res., 2009; 105: 1031-1040
Suschek C.V., Opländer C., van Faassen E.E.: Nonenzymatic NO production in human skin: effect of UVA on cutaneous NO stores. Nitric Oxide, 2010; 22: 120-135
Morcos E., Carlsson S., Weitzberg E., Wiklund N.P., Lundberg J.O.: Inhibition of cancer cell replication by inorganic nitrite. Nutr. Cancer, 2010; 62: 501-504
Schwarz T.: UV light affects cell membrane and cytoplasmic targets. J. Photochem. Photobiology B: Biology, 1998; 44: 91-96
Somosy Z.: Radiation response of cell organelles. Micron, 2000; 31: 165–181
Schwarz A., Bhardwaj R., Aragane Y.: Ultraviolet-B-induced apoptosis of keratinocytes: evidence for partial involvement of tumor necrosis factor-alpha in the formation of sunburn cells. J Invest Dermatol. 1995; 104: 922-927
Rehemtulla A., Hamilton Ch.A., Chinnaiyan A.M., Dixit V.M.: Ultraviolet Radiation-induced apoptosis is mediated by activation of CD-95 (Fas/APO-1), 1997; J. Biol. Chem., 1997; 41: 25783–25786
Kulms D., DuÈûmann H., Pöppelmann B., Ständer S., Schwarz, Schwarz T.: Apoptosis induced by disruption of the actin cytoskeleton is mediated via activation of CD95 (Fas/APO-1). Cell Death and Differentiation, 2002; 9: 598- 608
Grzanka D., Domaniewski J., Grzanka A., Żuryń A.: Ultraviolet radiation (UV) induces reorganization of actin cytoskeleton in CHOAA8 cells. Neoplsma, 2006; 53: 328- 332
Roninson I.B., Broude E.V., Chang B.D.: If not apoptosis, then what? Treatment-induced senescence and mitotic catastrophe in tumor cells. Drug Resist. Update 2001; 4: 303-313
Castedo M., Perfettini J.L., Roumier T., Andreau K., Medema R., Kroemer G.: Cell death by mitotic catastrophe: a molecular definition. Oncogene, 2004; 23: 2825-2837
Chen L-H., Chu P.M., Lee Y.J., et al.: Targeting Protective Autophagy Exacerbates UV-Triggered Apoptotic Cell Death. Int. J. Mol. Sci., 2012; 13: 1209-1224
Kondo Y., Kanzawa T., Sawaya R., et al.: The role of autophagy in cancer development and response to therapy. Nat. Rev. Cancer, 2005; 5: 726-734
Schweichel J.U., Merkel H.J.: The morphology of various types of cell death in prenatal tissue. Teratology, 1973; 7: 253–266
Maiuri M.C., Zalckvar E., Kimchi A., et al.: Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat. Rev. Mol. Cell Biol., 2007; 8: 741–752
Veselska R., Janisch R.: The effect of UV irradiation on changes in cytoskeleton and viability of mouse fibroblasts l929 cell line. Scripta Medica, 2000; 73(6): 393–408
Weinreb O., Dovrat A., Dunia I., et al.: UV-A-related alterations of young and adult lens water-insoluble alphacrystallin, plasma membranous and cytoskeletal proteins. Eur. J. Biochem., 2001; 268: 536-543
Desouza M., Gunning P.W., Stehn J.R.: The actin cytoskeleton as a sensor and mediator of apoptosis. BioArchitecture, 2012; 2: 75-87
Grzanka A., Grzanka D., Orlikowska M.: Cytoskeletal reorganization during process of apoptosis induced by cytostatic drugs in K-562 and HL-60 leukemia cell lines. Biochem. Pharmacol., 2003; 66: 1611-1617
Grzanka A., Grzanka D., Orlikowska M.: Fluorescence and ultrastructural localization of actin distribution patterns in the nucleus of HL-60 and K-562 cell lines treated with cytostatic drugs. Oncol. Rep., 2004 ;11: 765-770
Grzanka D., Grzanka A., Izdebska M., Gackowska L., Stepien A., Marszalek A.: Actin reorganization in CHO AA8 cells undergoing mitotic catastrophe and apoptosis induced by doxorubicin. Oncol Rep., 2010; 23: 655-663
Nowak J.M., Grzanka A., Gagat M., et al.: The influence of cotinine on the non-small-cell lung cancer line A549. Post. Hig. Med. Dosw., 2009; 63: 1-7
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